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ZFIN ID: ZDB-FIG-100401-9
Christie et al., 2010 - Syk and Zap-70 function redundantly to promote angioblast migration. Developmental Biology   340(1):22-29 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: 20-25 somites
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: 20-25 somites

Fig. 3 sykHI:zap-70HI morphants resemble vegfa morphants and lack activated ERK. (A) sykHI:zap-70HI Tg(fli1a:EGFP)y1morphants have a complete suppression of ISV sprouting which is phenotypically similar to the ISV stalling observed in high dose vegfa morphants (B). While the artery of 20s wildtype (WT) embryos (arrow, C′) shows activated ERK (arrow, C″) comparable staged sykHI:zap-70HI morphants (D) show a strong downregulation of activated ERK (arrow D′ = artery, D″). The level of downregulation of activated ERK in the artery (arrow, E′) is similar to vegfaHI morphants (E, E″). C′, D′, E′ are enlargements of GFP signal in the boxed region of C, D, E, respectively. C″, D″, E″ are enlargements of the αERK-P immunohistochemistry in the boxed regions of C, D, and E respectively.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab7-mapk IHC y1Tg standard conditions 20-25 somites vascular cord
IHC y1Tg + MO1-syk + MO2-zap70 standard conditions 20-25 somites Not Detected vascular cord
IHC y1Tg + MO2-vegfaa standard conditions 20-25 somites Not Detected vascular cord
Phenotype Details
Fish Conditions Stage Phenotype
y1Tg + MO1-syk + MO2-zap70 standard conditions 20-25 somites intersegmental vessel aplastic, abnormal
y1Tg + MO2-vegfaa standard conditions 20-25 somites intersegmental vessel aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 340(1), Christie, T.L., Carter, A., Rollins, E.L., and Childs, S.J., Syk and Zap-70 function redundantly to promote angioblast migration, 22-29, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.