ZFIN ID: ZDB-FIG-100319-25
Feng et al., 2010 - Pivotal role of hmx2 and hmx3 in zebrafish inner ear and lateral line development. Developmental Biology   339(2):507-518 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Prim-5 to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Prim-5 to Day 4

Fig. 3 Reduced number of hair cells and progressive fusion of two maculae in hmx2/hmx3 morphants. Anterior to the left, dorsal view in panels A–L and lateral view in panels M–R. (A–F) Hair cells are labeled with antibodies for S-100 (red) and acetylated-tubulin (green) at the stages indicated showing progressive fusion of the anterior (utricular) maculae with the posterior (saccular) maculae at the medial wall in the morphants. (G–N) In situ hybridization with Na,K-ATPase β2b probe to reveal mature hair cells, showing a reduced number in the anterior maculae (amac) and fusion with the posterior maculae (pmac). (O–R) Rhodamine–phalloidin staining of the F-actin of the hair bundles in control (O, Q) and morphants (P, R). (S) Illustration of control amac and pmac from 9 ears. (T) Illustration of fused maculae in morphants from 8 ears. Arrowheads indicate hair cells. Scale bars: (A–F) 50 μm, (G–R) 25 μm. Abbreviations: AM, anterior maculae; PM, posterior maculae; M, maculae fusion.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atp1b2b AB control Prim-5 anterior macula ISH
Prim-5 posterior macula ISH
Prim-15 anterior macula ISH
Prim-15 posterior macula ISH
Long-pec anterior macula ISH
Long-pec posterior macula ISH
AB + MO1-hmx2 + MO2-hmx3a standard conditions Prim-5 anterior macula ISH
Prim-5 posterior macula ISH
Prim-15 anterior macula ISH
Prim-15 posterior macula ISH
Long-pec macula ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-s100 IHC AB control Prim-5 anterior macula
IHC Prim-5 posterior macula
IHC Prim-15 anterior macula
IHC Prim-15 posterior macula
IHC Long-pec anterior macula
IHC Long-pec posterior macula
IHC AB + MO1-hmx2 + MO2-hmx3a standard conditions Prim-5 anterior macula
IHC Prim-5 posterior macula
IHC Prim-15 anterior macula
IHC Prim-15 posterior macula
IHC Long-pec macula
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-hmx2 + MO2-hmx3a standard conditions Prim-5 anterior macula mislocalised posteriorly, abnormal
Prim-5 hair cell anterior macula decreased amount, abnormal
Prim-5 tether cell morphology, normal
Prim-15 anterior macula mislocalised posteriorly, abnormal
Prim-15 hair cell anterior macula decreased amount, abnormal
Long-pec anterior macula fused with posterior macula, abnormal
Long-pec macula neuromast hair cell decreased amount, abnormal
Day 4 anterior macula fused with posterior macula, abnormal
Day 4 macula neuromast hair cell decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 339(2), Feng, Y., and Xu, Q., Pivotal role of hmx2 and hmx3 in zebrafish inner ear and lateral line development, 507-518, Copyright (2010) with permission from Elsevier. Full text @ Dev. Biol.