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ZFIN ID: ZDB-FIG-091221-2
Schwend et al., 2009 - Zebrafish con/disp1 reveals multiple spatiotemporal requirements for Hedgehog-signaling in craniofacial development. BMC Developmental Biology   9:59 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Terms:
Stage Range: 20-25 somites to Protruding-mouth

Fig. 2 CNCC patterning in the con/disp1 mutant is visualized by the fli1gfp transgene. Lateral views (A,D) or ventral views (B,C,E-L) of confocal stack projections of fli1GFP+ CNCC in wild type and con/disp1 mutants (A-H, J,K) or Alcian blue-stained larvae to visualize cartilages (I,L). (G,J) Zn5 (red) staining to visualize endodermal pouches (P 1-5) in fli1GFP embryos. (A,D) At 21 hpf, wild type (A) and con/disp1 mutants (D) reveal similar patterns of postmigratory-CNCC. (B,C) 32 hpf ventral views of wild type embryos reveal CNC condensations in PA (B), and segmentation of CNCC by 48 hpf (C). (E,F) By 32 hpf, con/disp1 anterior-most CNCC (asterisks) become mispatterned, while CNCC within more PA 2-7 are correctly patterned (E) and segmented by 48 hpf (F). (G,J) CNCC in wild type (G) and con/disp1 mutants (J) are interdigitated properly in well-formed endodermal pouches 1-5 (P 1-5) (Zn5, red). (H,K) con/disp1 mutants show developed, segmented CNCC in posterior arches (K). (I,L) CNCC within con/disp1 mutant posterior arches fail to become cartilage. Scale bar: 50 μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
EGFP disp1tm15a/tm15a; y1Tg standard conditions 20-25 somites pharyngeal arch cranial neural crest cell IFL
Prim-15 pharyngeal arch cranial neural crest cell IFL
Long-pec pharyngeal arch cranial neural crest cell IFL
Protruding-mouth pharyngeal arch cranial neural crest cell IFL
y1Tg standard conditions 20-25 somites pharyngeal arch cranial neural crest cell IFL
Prim-15 pharyngeal arch cranial neural crest cell IFL
Long-pec pharyngeal arch cranial neural crest cell IFL
Protruding-mouth pharyngeal arch cranial neural crest cell IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
zn-5 IHC disp1tm15a/tm15a; y1Tg standard conditions Long-pec pharyngeal pouch
IHC y1Tg standard conditions Long-pec pharyngeal pouch
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.