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ZIRC
ZFIN ID: ZDB-FIG-091217-57
Hogan et al., 2009 - Vegfc/Flt4 signalling is suppressed by Dll4 in developing zebrafish intersegmental arteries. Development (Cambridge, England)   136(23):4001-4009 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 1 Zebrafish expando mutants lack lymphatic vessels and venous sprouting. (A,B) Lateral views of TG(fli1:GFP) expression in wild-type (wt; A) and expando mutant (B) larvae at 5 dpf demonstrate that overall vascular patterning is not perturbed in expando mutants. (C,D) Overlay image of angiogram (red) and TG(fli1:GFP) (white) indicating the presence of blood flow in wild-type (C) and mutant (D) embryos. The thoracic duct (arrows) is present in the wild type, but absent (asterisks) from mutant larvae. (E,F) Lateral views of wild type (E) and expando mutant (F) in a double-transgenic TG(fli1:GFP), TG(kdr-l:Cherry) background. All lymphatic vessels are absent in mutants at 5 dpf. Arrows indicate the thoracic duct (td), intersegmental lymphatic vessels (islv) and dorsal longitudinal lymphatic vessels (dllv) in wild-type embryos. Asterisks indicate their absence in expando mutants. (G,H) Parachordal lymphangioblasts (PLs) are absent from the horizontal myoseptum in expando mutants (asterisk in H) as compared with wild-type siblings (arrow in G indicates PL) in the double-transgenic TG(fli1:GFP), TG(kdr-l:Cherry) background at 54 hpf. (I,J) tie2 expression in venous sprouts (arrows in I) is absent in expando mutants (J). n=6/29 embryos scored and confirmed by genotyping.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP flt4hu4602/hu4602; y1Tg standard conditions Day 5 caudal vein IFL
Day 5 dorsal longitudinal anastomotic vessel IFL
Day 5 intersegmental vessel IFL
Day 5 parachordal vessel IFL
y1Tg standard conditions Day 5 caudal vein IFL
Day 5 dorsal longitudinal anastomotic vessel IFL
Day 5 intersegmental vessel IFL
Day 5 parachordal vessel IFL
Day 5 thoracic duct IFL
mCherry flt4hu4602/hu4602; s916Tg; y1Tg standard conditions Day 5 Not Detected trunk lymph vasculature IFL
Day 5 trunk lymph vasculature IFL
s916Tg; y1Tg standard conditions Day 5 trunk lymph vasculature IFL
tek WT standard conditions Long-pec caudal vein plexus ISH
Long-pec intersegmental vessel ISH
flt4hu4602/hu4602 standard conditions Long-pec caudal vein plexus ISH
Long-pec Not Detected intersegmental vessel ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
flt4hu4602/hu4602; s916Tg; y1Tg standard conditions Day 5 lymph vessel development disrupted, abnormal
Day 5 trunk lymph vasculature aplastic, abnormal
flt4hu4602/hu4602; y1Tg standard conditions Day 5 lymph vessel development disrupted, abnormal
Day 5 thoracic duct aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development