ZFIN ID: ZDB-FIG-091214-5
Bibliowicz et al., 2009 - Expanded progenitor populations, vitreo-retinal abnormalities, and Muller glial reactivity in the zebrafish leprechaun/patched2 retina. BMC Developmental Biology   9:52 Full text @ BMC Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Term:
Stage: Day 5
PHENOTYPE:
Fish:
Observed In:
Stage: Day 5

Fig. 5 lep/ptc2 mutants display Müller glial reactivity and morphological abnormalities in the ILM. Immunohistochemical analysis using glutamine synthetase (GS) antibody, which marks differentiated Müller glia and their endfeet at the ILM, highlights disruptions in the ILM. (A) In sibling retinas, the ILM is tight and continuous (inset). (B) In lep/ptc2 retinas the ILM is discontinuous and Müller glial endfeet do not terminate properly at the ILM (inset). Glial fibrillary acidic protein (GFAP) antibody staining reveals elevated immuno-reactivity in the inner retina, adjacent to the optic nerve of lep/ptc2 (D and arrows in D′) mutant retinas as compared to siblings (C, C′). Approximately 40% of analyzed mutants displayed significant ILM disruptions and elevated GFAP levels.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
gfap zrf-1 WT standard conditions Day 5 Muller cell IHC
zrf-1 ptch1tj222/tj222(AB) standard conditions Day 5 Muller cell IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-glul IHC WT standard conditions Day 5 Muller cell
IHC ptch1tj222/tj222(AB) standard conditions Day 5 Muller cell
Phenotype Details
Fish Conditions Stage Phenotype
ptch1tj222/tj222(AB) standard conditions Day 5 inner limiting membrane broken, abnormal
ptch1tj222/tj222(AB) standard conditions Day 5 inner limiting membrane disorganized, abnormal
ptch1tj222/tj222(AB) standard conditions Day 5 iris increased size, abnormal
ptch1tj222/tj222(AB) standard conditions Day 5 Muller cell axon terminus physical object quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal BMC Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ BMC Dev. Biol.