FIGURE

Fig. 3

ID

ZDB-FIG-091121-3

Publication

Pittman et al., 2008 - Pathfinding in a large vertebrate axon tract: isotypic interactions guide retinotectal axons at multiple choice points

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Fig. 3

ath5 MO does not appear to affect the molecular and cellular environment of the optic nerve head or the intraretinal optic nerve. Coronal sections through 48-49 hpf isl2b:GFP (A,B) and 36 hpf non-transgenic (C,D) eyes, from either uninjected embryos (A,C) or embryos injected with high dose ath5 TMO (B,D). (A,B) Pax2a antibody staining (magenta) labels presumptive glial cells which line the intraretinal region of the optic nerve (arrows) in both wild type (A) and ath5 morphants (B). RGCs and their axons (green) are present in wild type (A), but not in ath5 morphants (B). The trigeminal ganglion (arrowheads) is also labeled by isl2b:gfp and serves as a staining control. (C,D) In situ hybridization shows that netrin1a expression (arrows) surrounds the optic nerve and optic nerve head in wild type (C), and is unchanged in ath5 morphants (D). Scale bars: 50 μm.

Expression Data

Genes:	GFP ntn1a pax2a
Fish:	WT WT + MO1-atoh7 zc7Tg zc7Tg + MO1-atoh7
Knockdown Reagent:	MO1-atoh7
Anatomical Terms:	glial cell optic nerve head retinal ganglion cell trigeminal ganglion
Stage Range:	Prim-25 to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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