header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-090817-26
Moro et al., 2009 - Analysis of beta cell proliferation dynamics in zebrafish. Developmental Biology   332(2):299-308 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Antibodies:
Fish:
Anatomical Terms:
Stage: Adult

Fig. 1 (A) Representative histology of pancreatic and gut tissue immunostained with antibodies against insulin (green) and BrdU (red). As expected, criptae of the gut, at the bottom, are strongly positive for BrdU. (B, C, E) Pancreatic islets from 9 months (B and E) and 5 months old fish (C) immunostained for insulin (green) and BrdU (red) and counterstained with DAPI (blue). White arrowheads indicate insulin and BrdU copositive cells. (D) Proliferation of beta cells, although at a low rate, was also confirmed by PCNA immunostaining as indicated by the arrowhead. (F) Histology of proliferating alpha/delta cells within an islet stained with antibodies against glucagon and somatostatin (cytoplasmic red). A white arrowhead indicates a glucagon/somatostatin producing cell copositive for BrdU (light nuclear red). (G) Representative pancreatic section showing a proliferating hormone negative cells (white arrowhead). Scale bar: 100 μm.

Gene Expression Details No data available
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-BrdU IHC WT standard conditions Adult endocrine pancreas
IHC Adult gut
Ab1-ins IHC WT standard conditions Adult endocrine pancreas
IHC Adult pancreas
Ab2-gcg IHC WT standard conditions Adult pancreatic A cell
Ab2-pcna IHC WT standard conditions Adult pancreatic B cell
Ab3-sst IHC WT standard conditions Adult pancreas D cell
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 332(2), Moro, E., Gn├╝gge, L., Braghetta, P., Bortolussi, M., and Argenton, F., Analysis of beta cell proliferation dynamics in zebrafish, 299-308, Copyright (2009) with permission from Elsevier. Full text @ Dev. Biol.