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ZFIN ID: ZDB-FIG-090804-73
Dodd et al., 2009 - The ENTH domain protein Clint1 is required for epidermal homeostasis in zebrafish. Development (Cambridge, England)   136(15):2591-2600 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: Long-pec to Day 5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage Range: Long-pec to Day 5

Fig. 6 Epidermal aggregation in clint1 mutants and interaction with lgl2. (A-D′) Wild-type (A-D) and clint1 mutant (A′-D′) zebrafish embryos staged at 48 (A,A′), 72 (B,C,B′,C′) and 120 (D,D′) hpf and immunolabeled for cadherin (green) and p63 (red) (A,A′) or for keratin (red) (B-D,B′-D′). Arrows (A′-D′) identify keratinocyte aggregation. Arrowheads (C,D′) identify keratin expression within fin folds. (E-H)p63 expression highlights epidermal morphology in uninjected (E), clint1-ex1 plus control MO (F), lgl2 plus control MO (G), or clint1-ex1 plus lgl2 MO (H) co-injected wild-type embryos. (I) Quantification of morphological phenotypes observed in E-H. Scale bar: 50 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
tp63 Ab1-tp63 AB control Long-pec epidermis IHC
Ab1-tp63 AB standard conditions Long-pec epidermis IHC
Ab1-tp63 AB + MO1-llgl2 standard conditions Long-pec epidermis IHC
Ab1-tp63 AB + MO1-llgl2 + MO2-clint1a standard conditions Long-pec epidermis IHC
Ab1-tp63 AB + MO2-clint1a standard conditions Long-pec epidermis IHC
Ab1-tp63 clint1ahi1520Tg/hi1520Tg(AB) standard conditions Long-pec epidermis IHC
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-krt IHC AB standard conditions Protruding-mouth epidermis keratinocyte
IHC Day 5 epidermis keratinocyte
IHC clint1ahi1520Tg/hi1520Tg(AB) standard conditions Protruding-mouth epidermis keratinocyte
IHC Day 5 epidermis keratinocyte
Ab1-pan-Cadherin IHC AB standard conditions Long-pec epidermis
IHC clint1ahi1520Tg/hi1520Tg(AB) standard conditions Long-pec epidermis
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO1-llgl2 + MO2-clint1a standard conditions Long-pec epidermis malformed, abnormal
Long-pec ventral fin fold malformed, abnormal
clint1ahi1520Tg/hi1520Tg(AB) standard conditions Protruding-mouth epidermis malformed, abnormal
Protruding-mouth ventral fin fold malformed, abnormal
Day 5 epidermis malformed, abnormal
Day 5 ventral fin fold malformed, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development