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ZFIN ID: ZDB-FIG-090428-6
Faro et al., 2009 - T-Cell Factor 4 (tcf7l2) Is the Main Effector of Wnt Signaling During Zebrafish Intestine Organogenesis. Zebrafish   6(1):59-68 Full text @ Zebrafish
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Protruding-mouth

Fig. 6 Depletion of either Tcf1 or Tcf3 in apcmcr/mcr genetic background does not rescue expression of differentiation markers in endoderm-derived tissues, but combined depletion recovers expression of l-fabp. Dorsal view of 72 hpf of apcmcr/mcr (A, E, I), apcmcr/mcr;tcf1hu3238/hu3238 (B, F, J), apcmcr/mcr;tcf3MO (C, G, K), and apcmcr/mcr;tcf1hu3238/hu3238;tcf3MO (D, H, L) after WISH for i-fabp (A–D), trypsin (E–H), and l-fabp (I–L).

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
fabp2 apchu745/hu745 standard conditions Protruding-mouth Not Detected intestinal epithelium ISH
apchu745/hu745 + MO1-tcf7l1a standard conditions Protruding-mouth Not Detected intestinal epithelium ISH
apchu745/hu745; tcf7hu3238/hu3238 standard conditions Protruding-mouth Not Detected intestinal epithelium ISH
apchu745/hu745; tcf7hu3238/hu3238 + MO1-tcf7l1a standard conditions Protruding-mouth Not Detected intestinal epithelium ISH
fabp10a apchu745/hu745 standard conditions Protruding-mouth Not Detected liver ISH
apchu745/hu745 + MO1-tcf7l1a standard conditions Protruding-mouth Not Detected liver ISH
apchu745/hu745; tcf7hu3238/hu3238 standard conditions Protruding-mouth Not Detected liver ISH
apchu745/hu745; tcf7hu3238/hu3238 + MO1-tcf7l1a standard conditions Protruding-mouth hepatocyte ISH
prss1 apchu745/hu745 standard conditions Protruding-mouth exocrine pancreas ISH
apchu745/hu745 + MO1-tcf7l1a standard conditions Protruding-mouth exocrine pancreas ISH
apchu745/hu745; tcf7hu3238/hu3238 standard conditions Protruding-mouth exocrine pancreas ISH
apchu745/hu745; tcf7hu3238/hu3238 + MO1-tcf7l1a standard conditions Protruding-mouth exocrine pancreas ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
apchu745/hu745 standard conditions Protruding-mouth endodermal digestive tract morphogenesis disrupted, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth hepatocyte differentiation disrupted, abnormal
apchu745/hu745 + MO1-tcf7l1a standard conditions Protruding-mouth endodermal digestive tract morphogenesis disrupted, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth hepatocyte differentiation disrupted, abnormal
apchu745/hu745; tcf7hu3238/hu3238 standard conditions Protruding-mouth endodermal digestive tract morphogenesis disrupted, abnormal
Protruding-mouth exocrine pancreas development disrupted, abnormal
Protruding-mouth hepatocyte differentiation disrupted, abnormal
apchu745/hu745; tcf7hu3238/hu3238 + MO1-tcf7l1a standard conditions Protruding-mouth endodermal digestive tract morphogenesis disrupted, abnormal
apchu745/hu745; tcf7hu3238/hu3238 + MO1-tcf7l1a standard conditions Protruding-mouth exocrine pancreas development disrupted, abnormal
apchu745/hu745; tcf7hu3238/hu3238 + MO1-tcf7l1a standard conditions Protruding-mouth hepatocyte differentiation process quality, normal
Acknowledgments:
ZFIN wishes to thank the journal Zebrafish for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Zebrafish