header logo image header logo text
Downloads Login
Research
General Information
ZIRC
ZFIN ID: ZDB-FIG-090223-30
Jin et al., 2009 - Definitive hematopoietic stem/progenitor cells manifest distinct differentiation output in the zebrafish VDA and PBI. Development (Cambridge, England)   136(4):647-654 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibody:
Fish:
Anatomical Term:
Stage Range: Long-pec to Protruding-mouth

Fig. 4 Definitive myeloid cells are generated in the VDA. (A) Lateral view of 21 hpf embryo, indicating the uncaging position (blue cross) in the anterior part of the ICM. (B,C) Lateral view of 2 dpf (B) and 3 dpf (C) embryos. The boxed regions indicate the relative positions in the VDA where flu and L-plastin protein double-positive cells were detected after uncaging. (D,E) Confocal images of the boxed region in B showing the flu signal and L-plastin staining in the VDA at 2 dpf after uncaging at cross in A. (D/E) Merged image of D and E. (D′/E′) Superimposed view of D/E and DIC image. (F,G) Confocal images of the boxed region in C showing the flu signal and L-plastin staining in the VDA at 3 dpf after uncaging at cross in A. (F/G) Merged image of F and G. (F′/G′) Superimposed view of F/G and DIC image. Staining for flu and L-plastin is pseudopainted as green and red, respectively. Arrows indicate co-staining of flu and L-plastin.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lcp1 Ab3-lcp1 y1Tg control Long-pec ventral wall of dorsal aorta myeloid cell IHC
Ab3-lcp1 Protruding-mouth ventral wall of dorsal aorta myeloid cell IHC
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development