FIGURE

Fig. 2

ID

ZDB-FIG-090223-18

Publication

Hinits et al., 2009 - Differential requirements for myogenic regulatory factors distinguish medial and lateral somitic, cranial and fin muscle fibre populations

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Fig. 2

myf5^hu2022 mutants appear normal as embryos but fail to mature into adults. (A) Myf5 truncation predicted from the myf5^hu2022 allele. The location of the introduced stop codon is shown; B, basic domain; HLH, helix-loop-helix domain. (B) Genotyped zebrafish embryos from a myf5^+/hu2022 carrier incross show no phenotype at 5 days post-fertilisation (5d) as compared with the wild type (+/+). (C) Survival at various ages of fish of each genoptype obtained from myf5^+/hu2022 incrosses. The numbers of embryos are shown. (D) In situ mRNA hybridisation for myf5 revealed mildly reduced signal in putative myf5^+/hu2022 (13/28) and lower levels in putative myf5^hu2022^/^hu2022 (9/28) embryos. No such effect was observed in myf5 morphants (0/27). (E) Embryos from an myf5^+/hu2022 incross lack adaxial myog mRNA early (15/49), and later have a mild delay in myog mRNA accumulation in fast muscle precursors correlated with reduced myf5 mRNA (20/74). Scale bars: 100 μm in D; 150 μm in E.

Expression Data

Genes:	myf5 myog
Fish:	WT myf5^{hu2022/hu2022} WT + MO3-myf5 myf5^hu2022/+
Knockdown Reagent:	MO3-myf5
Anatomical Terms:	adaxial cell segmental plate somite
Stage Range:	5-9 somites to 14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	myf5^{hu2022/hu2022}
Observed In:	whole organism
Stage Range:	Day 5 to Adult

Phenotype Detail

Acknowledgments

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