ZFIN ID: ZDB-FIG-081106-3
Rotllant et al., 2008 - Sparc (Osteonectin) functions in morphogenesis of the pharyngeal skeleton and inner ear. Matrix biology : journal of the International Society for Matrix Biology   27(6):561-572 Full text @ Matrix Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes: col2a1a, dlx2a, sox9a, sox9b
Fish: AB, AB + MO3-sparc
Knockdown Reagent: MO3-sparc
Anatomical Terms: chondrocranium cartilage, chondrocyte, cranial cartilage, cranial neural crest cell, mandibular arch skeleton (all 10) expand
Stage Range: Prim-5 to Protruding-mouth
PHENOTYPE:
Fish: AB + MO3-sparc
Knockdown Reagent: MO3-sparc
Observed In: ceratobranchial cartilage, ceratohyal cartilage, chondrocranium cartilage, cranial cartilage, Meckel's cartilage (all 10) expand
Stage Range: Protruding-mouth to Day 4

Fig. 4 ZFIN is incorporating published figure images and captions as part of an ongoing project. Figures from some publications have not yet been curated, or are not available for display because of copyright restrictions.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
col2a1a AB control Protruding-mouth chondrocyte ISH
Protruding-mouth cranial cartilage ISH
Protruding-mouth pectoral fin cartilage ISH
AB + MO3-sparc standard conditions Protruding-mouth chondrocyte ISH
Protruding-mouth cranial cartilage ISH
Protruding-mouth pectoral fin cartilage ISH
dlx2a AB control Prim-5 chondrocranium cartilage ISH
Prim-5 cranial neural crest cell ISH
Prim-5 mandibular arch skeleton ISH
Prim-5 pharyngeal arch ISH
Prim-5 pharyngeal arch 2 skeleton ISH
AB + MO3-sparc standard conditions Prim-5 chondrocranium cartilage ISH
Prim-5 cranial neural crest cell ISH
Prim-5 mandibular arch skeleton ISH
Prim-5 pharyngeal arch ISH
Prim-5 pharyngeal arch 2 skeleton ISH
sox9a AB control Prim-5 chondrocranium cartilage ISH
Prim-5 otic vesicle ISH
Prim-5 pharyngeal arch ISH
AB + MO3-sparc standard conditions Prim-5 chondrocranium cartilage ISH
Prim-5 otic vesicle ISH
Prim-5 pharyngeal arch ISH
sox9b AB control Prim-5 chondrocranium cartilage ISH
Prim-5 rhombomere ISH
AB + MO3-sparc standard conditions Prim-5 chondrocranium cartilage ISH
Prim-5 rhombomere ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO3-sparc standard conditions Protruding-mouth cranial cartilage morphology, abnormal
Protruding-mouth pectoral fin cartilage morphology, abnormal
Day 4 ceratobranchial cartilage aplastic, abnormal
Day 4 ceratohyal cartilage aplastic, abnormal
Day 4 ceratohyal cartilage decreased size, abnormal
Day 4 chondrocranium cartilage decreased size, abnormal
Day 4 Meckel's cartilage aplastic, abnormal
Day 4 Meckel's cartilage decreased size, abnormal
Day 4 pectoral fin decreased size, abnormal
Day 4 pectoral fin kinked, abnormal
Day 4 ventral mandibular arch decreased size, abnormal
Day 4 vestibuloauditory system functionality, abnormal
Day 4 whole organism decreased length, abnormal
Full text @ Matrix Biol.