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ZIRC
ZFIN ID: ZDB-FIG-081007-34
Mudumana et al., 2008 - odd skipped related1 reveals a novel role for endoderm in regulating kidney versus vascular cell fate. Development (Cambridge, England)   135(20):3355-3367 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: 14-19 somites

Fig. S5 Intermediate mesoderm patterning in pax2/8 and scl morphants. Expression of scl (blue) and myoD (red) in control (A), and pax2a/pax8 double morphant (B) demonstrates that loss of Pax gene expression does not cause scl upregulation. Arrowheads indicate the area of strongest upregulation of scl seen in osr1 morphants. Expression of pax2a in control (C), scl morphant (D) similarly shows no large expansion of pax2a expression. Expression of pax2a in osr1 morphant (E) and osr1/scl double morphant (F) shows no rescue of pax2a expression. Arrowheads in C-F indicate the proximal nephron segment lost in osr1 morphants. The data indicate that Pax genes and scl are not involved in a mutual repression mechanism.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
myod1 AB/TU control 14-19 somites segmental plate ISH
14-19 somites somite ISH
AB/TU + MO3-pax2a + MO4-pax8 + MO5-pax8 standard conditions 14-19 somites segmental plate ISH
14-19 somites somite ISH
pax2a AB/TU control 14-19 somites intermediate mesoderm ISH
AB/TU + MO2-osr1 standard conditions 14-19 somites intermediate mesoderm ISH
AB/TU + MO2-osr1 + MO7-tal1 standard conditions 14-19 somites intermediate mesoderm ISH
AB/TU + MO7-tal1 standard conditions 14-19 somites intermediate mesoderm ISH
tal1 AB/TU control 14-19 somites anterior lateral plate mesoderm ISH
AB/TU + MO3-pax2a + MO4-pax8 + MO5-pax8 standard conditions 14-19 somites anterior lateral plate mesoderm ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development