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ZFIN ID: ZDB-FIG-080829-28
Liu et al., 2008 - K-ras/PI3K-Akt signaling is essential for zebrafish hematopoiesis and angiogenesis. PLoS One   3(8):e2850 Full text @ PLoS One
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Conditions:
Observed In:
Stage Range: Day 4 to Day 6

Fig. S8

PI3K inhibitor wortmannin or MEK inhibitor U0126 could induce hematopoietic and angiogenic defects similar to the defects induced by K-ras knock-down.

(A) Either wortmannin or U0126 treatment were able to cause the hematopoietic defects. These defects include empty heart, with no or few red blood cells inside heart (indicated by arrows in ii and iii, compared to wild type in i), reduced or lack of normal circulation and reduced number of circulating red blood cells (indicated by arrows in v and vi, compared to wild type in iv), and accumulation of blood cells in some sites away from the circulation (indicated by arrows in vii and viii). All embryos were observed at 4 dpf (days-post fertilization), lateral view, anterior to the left and dorsal to the top.

(B) o-Dianisidine staining for wortmannin or U0126 treated embryos, showing loss or reduction of hemoglobin positive cells overall, especially inside heart and in yolk sac (indicated by empty arrows and block arrows respectively in ii, iii, v and vi, compared to wild type embryos in i and iv). Except for grouped embryos, all other embryos are lateral view, anterior to the left and dorsal to the top. Embryos were observed at 6 dpf.

(C) Either wortmannin or U0126 treatment were able to cause angiogenic defects. Inhibitor treatment for fli1-GFP embryos resulted in disorganized blood vessels, including the missing segmental vessels and/or bearing ectopic vessel sprouts (indicated by arrows in i and ii), similar to the defects caused by K-ras knock-down. Embryos were observed at 4 dpf, lateral view, anterior to the left and dorsal to the top.

Gene Expression Details No data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT chemical treatment: U0126 Day 4 cardiovascular system nucleate erythrocyte absent, abnormal
Day 6 cardiovascular system nucleate erythrocyte absent, abnormal
WT chemical treatment: wortmannin Day 4 cardiovascular system nucleate erythrocyte absent, abnormal
Day 6 cardiovascular system nucleate erythrocyte absent, abnormal
y1Tg chemical treatment: U0126 Day 4 intersegmental vessel aplastic, abnormal
Day 4 intersegmental vessel brochidodromous, abnormal
Day 4 intersegmental vessel disorganized, abnormal
y1Tg chemical treatment: wortmannin Day 4 intersegmental vessel aplastic, abnormal
Day 4 intersegmental vessel brochidodromous, abnormal
Day 4 intersegmental vessel disorganized, abnormal
Acknowledgments:
ZFIN wishes to thank the journal PLoS One for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS One