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ZFIN ID: ZDB-FIG-080604-56
Elworthy et al., 2008 - Expression of multiple slow myosin heavy chain genes reveals a diversity of zebrafish slow twitch muscle fibres with differing requirements for Hedgehog and Prdm1 activity. Development (Cambridge, England)   135(12):2115-2126 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Anatomical Terms:
Stage: Day 4
PHENOTYPE:
Fish:
Observed In:
Stage: Day 4

Fig. S3 smyhc1 expression in the craniofacial muscles is prdm1 independent. (A) Lateral views of wild-type sib and ubo (prdm1)tp39 mutant head at 96 hpf with immunofluorescence to the general muscle MF20 antigen and cartilage Col2 II-II6B3 antigen. In the ubo (prdm1)tp39 mutant, perturbations to the craniofacial muscles are associated with the major loss of skeletal elements in the posterior head mutant. The ceratobranchial cartilage is absent, and the sternohyoidus and inferior obliquus form abnormal attachments to the posterior basicranial commissure cartilage instead of the cleithrum. (B) Ventrolateral views of wild type and nrd (prdm1)m805 mutants at 96 hpf with smyhc1 (1 to 249 bp), smyhc2 (1 to 155 bp) or smyhc3 (1 to 129 bp) ISH showing prdm1-independent expression. nrd (prdm1)m805 mutants lack posterior transversus ventralis muscles. Scale bars: 25 ┬Ám. Abbreviations: bc, basicranial commissure; cb, ceratobranchial; iob, inferior obliquus; sh, sternohyoidus; tv, transversus ventralis.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
smyhc1 WT standard conditions Day 4 cephalic musculature ISH
prdm1am805/m805 standard conditions Day 4 cephalic musculature ISH
smyhc2 WT standard conditions Day 4 cephalic musculature ISH
prdm1am805/m805 standard conditions Day 4 cephalic musculature ISH
smyhc3 WT standard conditions Day 4 cephalic musculature ISH
prdm1am805/m805 standard conditions Day 4 cephalic musculature ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab-MF20 IHC WT standard conditions Day 4 muscle
IHC prdm1atp39/tp39 standard conditions Day 4 muscle
Ab1-col2a IHC WT standard conditions Day 4 cranial cartilage
IHC prdm1atp39/tp39 standard conditions Day 4 cranial cartilage
Phenotype Details
Fish Conditions Stage Phenotype
prdm1am805/m805 standard conditions Day 4 ventral transverse aplastic, abnormal
prdm1atp39/tp39 standard conditions Day 4 cephalic musculature morphology, abnormal
Day 4 ceratobranchial cartilage aplastic, abnormal
Day 4 muscle attachment process quality, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development