ZFIN ID: ZDB-FIG-080502-26
Farooq et al., 2008 - Histone deacetylase 3 (hdac3) is specifically required for liver development in zebrafish. Developmental Biology   317(1):336-353 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage: Protruding-mouth
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Protruding-mouth

Fig. 9 Double knockdown of hdac1 and hdac3 leads to severe defects in embryonic development including liver and blood vessel. Double knockdown of hdac1 and hdac3 was performed by simultaneous injection of both MOs (hdac1 MO at 3 ng/embryo; hdac3 MO at 12 ng/embryo) into Tg(lfabp:RFP; elaA:EGFP) and Tg(fli-1:EGFP) embryos and was compared with the same quantity of 5 bp mismatch morpolinos (control, A–B). Liver (thick white arrow) was absent at 3 dpf and blood vessels (thin white arrow) were severely disrupted. Embryos were severely deformed in the double knockdown morphants (G, H) and they cannot survive beyond 3 dpf. All images were side view, with anterior to the left. Scale bar is 100 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
DsRed gz15Tg control Protruding-mouth liver IFL
gz15Tg + MO1-hdac1 standard conditions Protruding-mouth liver IFL
gz15Tg + MO1-hdac1 + MO1-hdac3 standard conditions Protruding-mouth Not Detected liver IFL
gz15Tg + MO1-hdac3 standard conditions Protruding-mouth liver IFL
EGFP y1Tg control Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-hdac1 standard conditions Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-hdac1 + MO1-hdac3 standard conditions Protruding-mouth intersegmental vessel IFL
y1Tg + MO1-hdac3 standard conditions Protruding-mouth intersegmental vessel IFL
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
gz15Tg + MO1-hdac1 standard conditions Protruding-mouth liver decreased size, abnormal
Protruding-mouth liver development paedomorphic growth, abnormal
gz15Tg + MO1-hdac1 + MO1-hdac3 standard conditions Protruding-mouth liver aplastic, abnormal
gz15Tg + MO1-hdac3 standard conditions Protruding-mouth liver decreased size, abnormal
Protruding-mouth liver development paedomorphic growth, abnormal
y1Tg + MO1-hdac1 + MO1-hdac3 standard conditions Protruding-mouth intersegmental vessel decreased size, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 317(1), Farooq, M., Sulochana, K.N., Pan, X., To, J., Sheng, D., Gong, Z., and Ge, R., Histone deacetylase 3 (hdac3) is specifically required for liver development in zebrafish, 336-353, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.