ZFIN ID: ZDB-FIG-080424-7
Wallace et al., 2003 - Unique and conserved aspects of gut development in zebrafish. Developmental Biology   255(1):12-29 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Prim-15 to Protruding-mouth
PHENOTYPE:
Fish:
Observed In:
Stage Range: Prim-15 to Protruding-mouth

Fig. 8 gata-5/fau is required for pharynx morphogenesis but not formation of the esophagus or gut. (A–D) 34 hpf. (A) Lateral view of nkx-2.3 expression within the fau branchial arches with corresponding histological section (B) and laminin immunostaining (C). (B) The fau pharyngeal endoderm appears normal in tissue sections (compare with Fig. 5B). (C) Laminin within the fau pharynx is normal compared with wild-type (D). (E–H) 50 hpf. (E) Lateral view of nkx-2.3 expression within the fau branchial arches with corresponding histological section (F) and laminin (green) and cadherin (red inset) immunostainings in fau (G) and wild-type (H) embryos. The epithelial bilayer within the pharyngeal endoderm that is normally present at 50 hpf (H) is not present in fau mutants (G). (I–U) 74 hpf. (I, P) Lateral view of nkx-2.3 expression within the branchial arches of fau (I) and wild-type (P) larvae with corresponding histological sections (fau: J, M, K, L; and wild-type: Q, R, S). The fau pharynx is duplicated (J; 18%, n = 58) or widened (M) compared with wild-type siblings (Q). In contrast, fau esophagus morphology (K) and differentiation (N) is normal compared with wild-type siblings (R, T). (L) The solitary 74-hpf fau intestine is smaller than that of wild-type siblings (S). Apical binding of a fluorescent lectin (soybean agglutinin) that normally appears after the gut forms is present in both fau (O) and wild-type (U) 74-hpf larvae. In all panels: ph, pharynx; p, branchial arches; e, esophagus; h, hindbrain; I, intestine.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
nkx2.3 WT standard conditions Protruding-mouth pharyngeal arch 3-7 ISH
gata5tm236a/tm236a standard conditions Prim-15 pharyngeal arch 3-7 ISH
Long-pec pharyngeal arch 3-7 ISH
Protruding-mouth pharyngeal arch 3-7 ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-lama1 IHC WT standard conditions Prim-15 pharyngeal endoderm
IHC Long-pec pharyngeal epithelium
IHC gata5tm236a/tm236a standard conditions Prim-15 pharyngeal endoderm
IHC Long-pec pharyngeal epithelium
Ab1-pan-Cadherin IHC WT standard conditions Prim-15 pharyngeal endoderm
IHC Long-pec pharyngeal epithelium
IHC gata5tm236a/tm236a standard conditions Prim-15 pharyngeal endoderm
IHC Long-pec pharyngeal epithelium
Phenotype Details
Fish Conditions Stage Phenotype
gata5tm236a/tm236a standard conditions Prim-15 pharyngeal endoderm morphology, normal
gata5tm236a/tm236a standard conditions Long-pec pharyngeal epithelium absent, abnormal
gata5tm236a/tm236a standard conditions Protruding-mouth esophagus morphology, normal
gata5tm236a/tm236a standard conditions Protruding-mouth intestine decreased size, abnormal
gata5tm236a/tm236a standard conditions Protruding-mouth pharynx increased amount, abnormal
gata5tm236a/tm236a standard conditions Protruding-mouth pharynx increased width, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 255(1), Wallace, K.N. and Pack, M., Unique and conserved aspects of gut development in zebrafish, 12-29, Copyright (2003) with permission from Elsevier. Full text @ Dev. Biol.