ZFIN ID: ZDB-FIG-080411-61
Webb et al., 2008 - psoriasis regulates epidermal development in zebrafish. Developmental dynamics : an official publication of the American Association of Anatomists   237(4):1153-1164 Full text @ Dev. Dyn.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Antibodies:
Fish:
Anatomical Terms:
Stage Range: Long-pec to Day 4
PHENOTYPE:
Fish:
Observed In:
Stage Range: Protruding-mouth to Day 4

Fig. 5 psoriasis mutants fail to express differentiation-specific keratins. A-D: Keratin expression in epidermal cells. Wild-type epidermal cells express and localize keratins basally at 3.5 (A) and 4.5 (C) days postfertilization (dpf), whereas psoriasis mutant epidermal cells have greatly decreased keratin expression at 3.5 (B) and 4.5 (D) dpf. The round green cells in B are unidentified, but are likely to be neuromast precursors based on regular spacing along the anterior-posterior axis in the position of the future lateral line. Cadherin staining (red) marks epidermal cell membranes. E,F: Keratin expression along the axis of the tail. Arrowheads indicate hypochord, and arrows indicate neural tube, respectively. Keratin expression is normal in this region of wild-type (E) and psoriasis mutants (F). E and F are lateral views with anterior to the upper left. G-J: In situ hybridizations for krt4. Wild-type embryos express krt4 at 48 hours postfertilization (hpf; G) and at lower levels at 3.5 dpf (I). psoriasis mutants express krt4 at 48 hpf similar to wild-type embryos (H), but exhibit increased krt4 expression at 3.5 dpf (J). Embryos in all these panels were stained for the same length of time. Anterior is to the left and dorsal is up in G-J. Scale bars = 25 μm in E,F, 50 μm in G-J.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
krt4 WT standard conditions Long-pec epidermis ISH
Protruding-mouth epidermis ISH
atp1b1am14/m14(AB) standard conditions Long-pec epidermis ISH
Protruding-mouth epidermis ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-krt IHC WT standard conditions Protruding-mouth epidermal cell basal region
IHC Protruding-mouth epidermis epidermal cell
IHC Day 4 epidermal cell basal region
IHC Day 4 epidermis epidermal cell
IHC atp1b1am14/m14(AB) standard conditions Protruding-mouth Not Detected epidermis epidermal cell
IHC Day 4 Not Detected epidermis epidermal cell
Ab1-pan-Cadherin IHC WT standard conditions Protruding-mouth epidermal cell membrane
IHC Day 4 epidermal cell membrane
IHC atp1b1am14/m14(AB) standard conditions Protruding-mouth epidermal cell membrane
IHC Day 4 epidermal cell membrane
Phenotype Details
Fish Conditions Stage Phenotype
atp1b1am14/m14(AB) standard conditions Protruding-mouth epidermis krt4 expression increased amount, abnormal
Protruding-mouth epidermis krt4 expression increased distribution, abnormal
Protruding-mouth epidermis epidermal cell ab1-krt labeling absent, abnormal
Protruding-mouth - Day 4 keratinocyte differentiation disrupted, abnormal
Day 4 epidermis epidermal cell ab1-krt labeling absent, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental dynamics : an official publication of the American Association of Anatomists for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Dev. Dyn.