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ZFIN ID: ZDB-FIG-080326-121
Bonner et al., 2008 - Proliferation and patterning are mediated independently in the dorsal spinal cord downstream of canonical Wnt signaling. Developmental Biology   313(1):398-407 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Term:
Stage: Prim-5
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Prim-5

Fig. 4 Tcf3 is required for proliferation but not for dorsal progenitor patterning. (A–D) Both tcf3a (A, B) and tcf3b (C, D) are expressed at higher levels in tcf7 morphants. (E) The mitotic index in dorsal, intermediate and ventral domains is significantly reduced in tcf3a/tcf3b double morphants. *p < 0.05 by T-test, error bars indicate ± SEM. (F, G) Expression of msxc and (H, I) pax3 is unaffected in tcf3a/tcf3b double morphants. (J, K) Expression of dbx2 is eliminated in tcf3a/tcf3b double morphants, but the intermediate progenitor domain is not shifted dorsally as indicated by iro3 expression (L, M). (A–D) 12 μm cross-sections are shown. (F–M) Lateral mounts are shown. Scale bars = 10 μm.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dbx2 AB standard conditions Prim-5 spinal cord ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions Prim-5 Not Detected spinal cord ISH
irx3a AB standard conditions Prim-5 spinal cord ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions Prim-5 spinal cord ISH
msx3 AB standard conditions Prim-5 spinal cord ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions Prim-5 spinal cord ISH
pax3a AB standard conditions Prim-5 spinal cord ISH
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions Prim-5 spinal cord ISH
tcf7l1a AB standard conditions Prim-5 spinal cord ISH
AB + MO1-tcf7 standard conditions Prim-5 spinal cord ISH
tcf7l1b AB standard conditions Prim-5 spinal cord ISH
AB + MO1-tcf7 standard conditions Prim-5 spinal cord ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
AB + MO2-tcf7l1a + MO3-tcf7l1b standard conditions Prim-5 cell population proliferation occurrence, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 313(1), Bonner, J., Gribble, S.L., Veien, E.S., Nikolaus, O.B., Weidinger, G., and Dorsky, R.I., Proliferation and patterning are mediated independently in the dorsal spinal cord downstream of canonical Wnt signaling, 398-407, Copyright (2008) with permission from Elsevier. Full text @ Dev. Biol.