ZFIN ID: ZDB-FIG-080325-55
Ke et al., 2008 - Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment. Molecular and cellular neurosciences   37(2):388-401 Full text @ Mol. Cell Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibody:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: 20-25 somites to Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. S2 Expression of segmentation markers in Gli2b morphants. (A, B) Expression of val in r5 and r6 in controls (A) and Gli2b morphants. (C, D) Expression of krox20 in r3 and r5 in controls (C) and Gli2b morphants (D). (E, F) Expression of hoxa2 in r2–r5 in controls (E) and Gli2b morphants (F). (G, H) Expression of hoxb3 in r5–7 in controls (G) and Gli2b morphants (H). (I, J) Immunohistochemical staining with anti-acetyl-tubulin antibody in controls (I) and Gli2b morphants (J).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
egr2b WT control 20-25 somites rhombomere 3 ISH
20-25 somites rhombomere 5 ISH
WT + MO1-gli2b standard conditions 20-25 somites rhombomere 3 ISH
20-25 somites rhombomere 5 ISH
hoxa2b WT control Prim-5 rhombomere 2 ISH
Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 4 ISH
Prim-5 rhombomere 5 ISH
WT + MO1-gli2b standard conditions Prim-5 rhombomere 2 ISH
Prim-5 rhombomere 3 ISH
Prim-5 rhombomere 4 ISH
Prim-5 rhombomere 5 ISH
hoxb3a WT control Prim-5 rhombomere 5 ISH
Prim-5 rhombomere 6 ISH
Prim-5 rhombomere 7 ISH
WT + MO1-gli2b standard conditions Prim-5 rhombomere 5 ISH
Prim-5 rhombomere 6 ISH
Prim-5 rhombomere 7 ISH
mafba WT control 20-25 somites rhombomere 5 ISH
20-25 somites rhombomere 6 ISH
WT + MO1-gli2b standard conditions 20-25 somites rhombomere 5 ISH
20-25 somites rhombomere 6 ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-tuba IHC WT control Long-pec hindbrain axon
IHC WT + MO1-gli2b standard conditions Long-pec hindbrain axon
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gli2b standard conditions Long-pec axonogenesis disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular and cellular neurosciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and cellular neurosciences, 37(2), Ke, Z., Kondrichin, I., Gong, Z., and Korzh, V., Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment, 388-401, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.