ZFIN ID: ZDB-FIG-080325-48
Ke et al., 2008 - Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment. Molecular and cellular neurosciences   37(2):388-401 Full text @ Mol. Cell Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 6 Gli2b and r4. nkx2.2 expression in the hindbrain in 30 hpf, dorsal view. (A) control, (B) Gli2b morphant. nkx2.2 expression domain was slightly expanded in Gli2b morphant (two-headed arrow). (C) yot-/- mutant. (D) yot-/-/Gli2b morphant without nkx2.2 expression in the hindbrain (black arrow). This phenotype is similar to that in smu-/- (E). (F) Dorsal and (H) lateral view of netrin1a expression in the hindbrain of 30 hpf controls and Gli2b morphants (G, I). In Gli2b morphants netrin1a expression in the ventral hindbrain domain was up-regulated and expanded laterally. (J) In yot-/-, netrin1a expression was affected ventrally, but unaffected dorsally. After injection of Gli2b MO into yot-/- only a trace of expression was detected in the r4 (black arrow, F). Black arrowhead in panel H—floor plate expression domain; white bullet line in panels H and J defines D-V extent of expression domain. Abbreviations: e, ear.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
nkx2.2a WT control Prim-15 hindbrain ISH
Prim-15 rhombomere ISH
WT + MO1-gli2b standard conditions Prim-15 hindbrain ISH
Prim-15 rhombomere ISH
gli2aty17a/ty17a control Prim-15 rhombomere 4 ISH
gli2aty17a/ty17a + MO1-gli2b standard conditions Prim-15 Not Detected hindbrain ISH
smob641/b641 standard conditions Prim-15 Not Detected hindbrain ISH
ntn1a WT control Prim-15 floor plate rhombomere region ISH
Prim-15 rhombomere ISH
WT + MO1-gli2b standard conditions Prim-15 floor plate rhombomere region ISH
gli2aty17a/ty17a control Prim-15 floor plate rhombomere 4 ISH
Prim-15 rhombomere ISH
gli2aty17a/ty17a + MO1-gli2b standard conditions Prim-15 rhombomere 4 ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gli2b standard conditions Prim-15 floor plate formation disrupted, abnormal
WT + MO1-gli2b standard conditions Prim-15 floor plate rhombomere region increased width, abnormal
gli2aty17a/ty17a standard conditions Prim-15 floor plate formation disrupted, abnormal
gli2aty17a/ty17a + MO1-gli2b standard conditions Prim-15 floor plate formation disrupted, abnormal
smob641/b641 standard conditions Prim-15 floor plate formation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular and cellular neurosciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and cellular neurosciences, 37(2), Ke, Z., Kondrichin, I., Gong, Z., and Korzh, V., Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment, 388-401, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.