ZFIN ID: ZDB-FIG-080325-45
Ke et al., 2008 - Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment. Molecular and cellular neurosciences   37(2):388-401 Full text @ Mol. Cell Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Long-pec
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Long-pec

Fig. 5 Gli2/Gli2b and cell differentiation. (A) Islet1-positive branchiomotor neurons (BMNs) and (B) RBs in 48 hpf wild-type embryos. Rhombomeres are numbered in Arabic. The motor nuclei of cranial nerves numbers are in Roman as follows: V, trigeminal; VI, abducens; VII, facial; X, vagal. (C) In Gli2b morphants the motor nuclei (in particular, the facial one, two-headed arrow) were further away from the ventral midline. (D) In Gli2b morphants the RBs were slightly reduced. (E) BMNs and (F) RBs in yot-/- mutant. The trigeminal and vagal motor nuclei are absent. Cell migration of the facial nucleus from rhombomere 4 to rhombomeres 6, 7 is affected (E). No major change in distribution of RBs was found in yot-/-. (G) The residual cells of facial nucleus of yot-/- were reduced even further after injection of gli2b MO while some that remain (arrows) migrated to rhombomeres 6, 7, and the number of RBs (arrowheads) was largely reduced (H). (I) The abducens nucleus (VI) labeled with zn5 antibody in 48 hpf wild-type embryos. These cells were lost in Gli2b morphants (J). (K, L) Position-related changes of motor neurons in controls (K) and Gli2b morphants (L). (M, N) Blow-up of the mid-trunk region indicated by the red line in panels K and L. Abbreviations: e, ear.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
alcama zn-5 WT control Long-pec abducens motor nucleus IHC
zn-5 Long-pec secondary motor neuron IHC
zn-5 WT + MO1-gli2b standard conditions Long-pec Not Detected abducens motor nucleus IHC
zn-5 Long-pec Not Detected secondary motor neuron IHC
GFP rw0Tg control Long-pec secondary motor neuron IFL
Long-pec spinal cord IFL
rw0Tg + MO1-gli2b standard conditions Long-pec secondary motor neuron IFL
Long-pec spinal cord IFL
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Qualifier Anatomy
Ab1-isl1 IHC WT control Long-pec facial nerve motor nucleus
IHC Long-pec Rohon-Beard neuron
IHC Long-pec trigeminal motor nucleus
IHC Long-pec vagal lobe
IHC WT + MO1-gli2b standard conditions Long-pec facial nerve motor nucleus
IHC Long-pec Rohon-Beard neuron
IHC Long-pec trigeminal motor nucleus
IHC Long-pec vagal lobe
IHC gli2aty17a/ty17a control Long-pec facial nerve motor nucleus
IHC Long-pec Rohon-Beard neuron
IHC Long-pec Not Detected trigeminal motor nucleus
IHC Long-pec Not Detected vagal lobe
IHC gli2aty17a/ty17a + MO1-gli2b standard conditions Long-pec facial nerve motor nucleus
IHC Long-pec Rohon-Beard neuron
IHC Long-pec Not Detected trigeminal motor nucleus
IHC Long-pec Not Detected vagal lobe
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gli2b standard conditions Long-pec abducens motor nucleus aplastic, abnormal
WT + MO1-gli2b standard conditions Long-pec facial nerve motor nucleus mislocalised laterally, abnormal
WT + MO1-gli2b standard conditions Long-pec Rohon-Beard neuron decreased amount, abnormal
WT + MO1-gli2b standard conditions Long-pec secondary motor neuron decreased amount, abnormal
WT + MO1-gli2b standard conditions Long-pec trigeminal motor nucleus mislocalised laterally, abnormal
gli2aty17a/ty17a standard conditions Long-pec facial nerve motor nucleus mislocalised anteriorly, abnormal
Long-pec trigeminal motor nucleus aplastic, abnormal
Long-pec vagal lobe aplastic, abnormal
gli2aty17a/ty17a + MO1-gli2b standard conditions Long-pec facial nerve motor nucleus decreased amount, abnormal
Long-pec Rohon-Beard neuron decreased amount, abnormal
Long-pec trigeminal motor nucleus aplastic, abnormal
Long-pec vagal lobe aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular and cellular neurosciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and cellular neurosciences, 37(2), Ke, Z., Kondrichin, I., Gong, Z., and Korzh, V., Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment, 388-401, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.