ZFIN ID: ZDB-FIG-080325-43
Ke et al., 2008 - Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment. Molecular and cellular neurosciences   37(2):388-401 Full text @ Mol. Cell Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Prim-5 to Day 4
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-5

Fig. 4 Gli2b and Olig2. olig2 expression in the hindbrain in 24 hpf control embryo in lateral view (A, black arrow), 24 hpf Gli2b morphant in lateral view (B), 24 hpf control embryo in dorsal view (C, black arrow) and 24 hpf smu mutant in lateral view (D). In the ET3 line GFP maps oligodendrocytes as shown by double staining of anti-GFP antibody and antisense mbp probes (E). Blow-up (F) of the black frame in panel E reveals the mbp mRNA (blue, arrows) in GFP expressing cells (brown). (G) 4 dpf control ET3 line and ET3 Gli2b morphants (J). Blow-up of the hindbrain (H) and posterior trunk (I) in 4 dpf ET3 controls. Blow-up of the hindbrain (K) and posterior trunk (L) in ET3-Gli2b morphant.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
EGFP sqet3Et control Day 4 hindbrain IFL
Day 4 trunk IFL
sqet3Et standard conditions Prim-5 neural tube IHC
Prim-5 oligodendrocyte IHC
sqet3Et + MO1-gli2b standard conditions Day 4 hindbrain IFL
Day 4 trunk IFL
mbpa sqet3Et standard conditions Prim-5 neural tube ISH
Prim-5 oligodendrocyte ISH
olig2 WT control Prim-5 hindbrain ISH
WT + MO1-gli2b standard conditions Prim-5 Not Detected hindbrain ISH
smob641/b641 standard conditions Prim-5 Not Detected hindbrain ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gli2b standard conditions Prim-5 oligodendrocyte differentiation disrupted, abnormal
smob641/b641 standard conditions Prim-5 oligodendrocyte differentiation disrupted, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular and cellular neurosciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and cellular neurosciences, 37(2), Ke, Z., Kondrichin, I., Gong, Z., and Korzh, V., Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment, 388-401, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.