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ZFIN ID: ZDB-FIG-080325-35
Ke et al., 2008 - Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment. Molecular and cellular neurosciences   37(2):388-401 Full text @ Mol. Cell Neurosci.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Antibodies:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage: Prim-15
PHENOTYPE:
Fish:
Knockdown Reagent:
Observed In:
Stage: Prim-15

Fig. 1 Gli2b and cell proliferation in the hindbrain. (A, B) zrf-1 stained radial glial cells in the hindbrain in 30 hpf control (A) and Gli2b morphant (B), dorsal view. (C, D) Expression of gfap in control (C) Gli2b morphant (D). (E, F) Cross sections of 30 hpf control (E) and Gli2b morphant (F) at the posterior hindbrain level stained by anti-P-H3 (red) and HuC (green) antibody. (G) A graph based on Table 1 that demonstrates changes in a number of proliferating cells in Gli2b morphants comparing to controls. Abbreviations: e, ear; pz, pial zone; vz, ventricular zone.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dnajc2 WT control Prim-15 astrocyte IHC
Prim-15 hindbrain IHC
WT + MO1-gli2b standard conditions Prim-15 astrocyte IHC
Prim-15 hindbrain IHC
gfap WT control Prim-15 astrocyte ISH
Prim-15 hindbrain ISH
WT + MO1-gli2b standard conditions Prim-15 astrocyte ISH
Prim-15 hindbrain ISH
Antibody Labeling Details
Antibody Assay Fish Conditions Stage Anatomy
Ab1-elavl IHC WT control Prim-15 hindbrain
IHC WT + MO1-gli2b standard conditions Prim-15 hindbrain
Ab1-h3 IHC WT control Prim-15 hindbrain
IHC WT + MO1-gli2b standard conditions Prim-15 hindbrain
zrf-1 IHC WT control Prim-15 astrocyte
IHC Prim-15 hindbrain
IHC WT + MO1-gli2b standard conditions Prim-15 astrocyte
IHC Prim-15 hindbrain
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-gli2b standard conditions Prim-15 hindbrain astrocyte decreased amount, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Molecular and cellular neurosciences for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Molecular and cellular neurosciences, 37(2), Ke, Z., Kondrichin, I., Gong, Z., and Korzh, V., Combined activity of the two Gli2 genes of zebrafish play a major role in Hedgehog signaling during zebrafish neurodevelopment, 388-401, Copyright (2008) with permission from Elsevier. Full text @ Mol. Cell Neurosci.