FIGURE

Fig. 2

ID
ZDB-FIG-080323-2
Publication
Londin et al., 2007 - Churchill regulates cell movement and mesoderm specification by repressing Nodal signaling
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Fig. 2

chch represses mesodermal markers. Mesodermal markers were examined by RNA in situ hybridization and real-time PCR in embryos microinjected with chch-EnR mRNA. Inhibition of chch does not alter expression of dorsal mesodermal genes chd (A, B) or flh (C, D). Expression of mesodermal markers at shield stage, including spt (E-F) and ntl (G-H) are expanded slightly in chch-inhibited embryos. Overexpression of chch mRNA does not alter no-tail expression at shield stage (I-J). chch inhibition results in a decrease in Sip1 gene expression at bud stage (M-N), while chch activation results in Sip1 induction at shield stage (K-L). Real-time PCR analysis of mesodermal markers in chch-inhibited embryos (O). The fold change in transcript levels (y-axis) is graphed relative to control embryos following overexpression of chch-EnR mRNA, chch-ATGMO and chch mRNA. This analysiinhibited embryos (O). The fold change in transcript levels (y-axis) is graphed relative to control embryos following overexpression of chch mRNA does not result in alteration of early mesodermal gene expression. The induction of ntl expression following chch inhibition with the ATGMO can be rescued by co-expression of chch mRNA (P). Real-time PCR analysis of Sip1 mRNA in chch, chch-EnR and chch-ATGMO treated embryos (Q). Induction of chch results in a 50% increase in Sip1 expression, conversely, inhibition of chch results in a 70% reduction of Sip1 expression. The fold change in Sip1 transcript levels (y-axis

Expression Data
Genes:
Fish:
Knockdown Reagent:
Anatomical Term:
Stage: Shield

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ BMC Dev. Biol.