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ZFIN ID: ZDB-FIG-070823-4
Inbal et al., 2007 - Six3 represses nodal activity to establish early brain asymmetry in zebrafish. Neuron   55(3):407-415 Full text @ Neuron
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage: 20-25 somites

Fig. 4 Six3 Misexpression Can Repress Diencephalic Nodal Activity If Induced by the Beginning of Somitogenesis Tg[hsp70l:Gal4-VP16]vu22;Tg[UAS-six3b]vu156 (B, D, and F) and single or nontransgenic siblings (A, C, and E), heat-shocked at tailbud (10 hpf) (A and B), 4s stage (C and D), or 6s stage (E and F). Arrowheads point at the left-sided dorsal diencephalic domain of Nodal activity represented by pitx2 expression. (G–J) Embryos from a cross between six3bvu87/+;Tg[hsp70l:Gal4-VP16]vu22 heterozygous and six3bvu87/+;Tg[UAS-six3b]vu156 heterozygous fish, which were injected with MOsix7 and heat-shocked at 80% epiboly (80%E) (G and H) or 4s stage (I and J). Insets are dorsoposterior view of the same embryo (A–F) or a representative embryo (G–I). L, left; R, right. Embryos are 22–24s stage. Genotypes in (G), (I), and (J) were inferred from clearly reduced eye size, demonstrating that embryos are six3b/six7 deficient. Because inducing six3b expression by heat shock at 80%E rescues the reduced eye size in six3b/six7-deficient embryos, embryos in (H) were PCR genotyped and the presence of all genotypes at expected ratios was confirmed.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
lft1 six3bvu87/vu87 + MO1-six7(AB) heat shock 20-25 somites epithalamus ISH
six3bvu87/vu87 + MO1-six7(AB) heat shock 20-25 somites epithalamus ISH
six3bvu87/vu87; vu156Tg; vu22Tg + MO1-six7 heat shock 20-25 somites epithalamus ISH
six3bvu87/vu87; vu156Tg; vu22Tg + MO1-six7 heat shock 20-25 somites Not Detected epithalamus ISH
six3bvu87; vu156Tg; vu22Tg + MO1-six7 heat shock 20-25 somites epithalamus ISH
pitx2 WT heat shock 20-25 somites epithalamus ISH
WT heat shock 20-25 somites epithalamus ISH
WT heat shock 20-25 somites epithalamus ISH
vu156Tg; vu22Tg heat shock 20-25 somites epithalamus ISH
vu156Tg; vu22Tg heat shock 20-25 somites epithalamus ISH
vu156Tg; vu22Tg heat shock 20-25 somites Not Detected epithalamus ISH
six3b WT heat shock 20-25 somites forebrain ISH
20-25 somites immature eye ISH
WT heat shock 20-25 somites forebrain ISH
20-25 somites immature eye ISH
WT heat shock 20-25 somites forebrain ISH
20-25 somites immature eye ISH
six3bvu87/vu87 + MO1-six7(AB) heat shock 20-25 somites forebrain ISH
20-25 somites immature eye ISH
six3bvu87/vu87 + MO1-six7(AB) heat shock 20-25 somites forebrain ISH
20-25 somites immature eye ISH
six3bvu87/vu87; vu156Tg; vu22Tg + MO1-six7 heat shock 20-25 somites whole organism ISH
six3bvu87/vu87; vu156Tg; vu22Tg + MO1-six7 heat shock 20-25 somites whole organism ISH
six3bvu87; vu156Tg; vu22Tg + MO1-six7 heat shock 20-25 somites whole organism ISH
vu156Tg; vu22Tg heat shock 20-25 somites whole organism ISH
vu156Tg; vu22Tg heat shock 20-25 somites whole organism ISH
vu156Tg; vu22Tg heat shock 20-25 somites whole organism ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neuron for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neuron