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ZFIN ID: ZDB-FIG-070823-3
Inbal et al., 2007 - Six3 represses nodal activity to establish early brain asymmetry in zebrafish. Neuron   55(3):407-415 Full text @ Neuron
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Bud to 20-25 somites

Fig. 3 Six3 Function Is Required in the Neuroectoderm to Repress Diencephalic Nodal Activity (A–C) Wild-type embryos at tailbud (10 hpf) (A), 2s (B), and 4s (C) stages, labeled for six3b and flh expression, which marks presumptive epithalamic and dorsal telencephalic tissues. Overlap between six3b and the flh-positive domain is indicated by arrows. (D–F) Embryos from a cross between six3bvu87/+;Tg[gsc:Gal4-VP16]vu160 heterozygous and six3bvu87/+;Tg[UAS-six3b]vu156 heterozygous fish. Control embryo (D) not injected with MOsix7. (E and F) six3b/six7-deficient embryos. Only the embryo in (F) carries both transgenes, as evidenced by ectopic six3b in the notochord (arrow). (G and H) Embryos from a cross between Tg[hsp70l:Gal4-VP16]vu22 and Tg[UAS-six3b]vu156 heterozygous fish that were injected with lft1 RNA and heat-shocked at late gastrulation (H). (I and J) Ubiquitous six3b does not eliminate presumptive pineal tissue (flh-positive domain, arrowheads in [I] and [J]). R, right; L, left. (A–C) Dorsal views, anterior up. (D–F, I, and J) Lateral views, anterior to the left. Insets in (D)-(H) are dorsoposterior views.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lft1 six3bvu87/vu87(AB) control 20-25 somites epithalamus ISH
20-25 somites notochord ISH
six3bvu87/vu87 + MO1-six7(AB) standard conditions 20-25 somites epithalamus ISH
20-25 somites notochord ISH
six3bvu87/vu87; vu156Tg; vu160Tg + MO1-six7 standard conditions 20-25 somites epithalamus ISH
20-25 somites notochord ISH
noto WT standard conditions Bud forebrain neural keel ISH
1-4 somites presumptive telencephalon ISH
WT heat shock 20-25 somites epiphysis ISH
vu156Tg; vu22Tg heat shock 20-25 somites epiphysis ISH
six3b WT standard conditions Bud forebrain neural keel ISH
1-4 somites forebrain neural keel ISH
WT heat shock 20-25 somites forebrain ISH
20-25 somites immature eye ISH
six3bvu87/vu87(AB) control 20-25 somites forebrain ISH
20-25 somites immature eye ISH
six3bvu87/vu87 + MO1-six7(AB) standard conditions 20-25 somites forebrain ISH
20-25 somites immature eye ISH
six3bvu87/vu87; vu156Tg; vu160Tg + MO1-six7 standard conditions 20-25 somites forebrain ISH
20-25 somites immature eye ISH
20-25 somites notochord ISH
vu156Tg; vu22Tg heat shock 20-25 somites whole organism ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Neuron for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Neuron