FIGURE

Fig. 4

ID

ZDB-FIG-070813-28

Publication

Etard et al., 2007 - The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis

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Fig. 4

Knockdown of Steif phenocopies the mutant phenotype. Two-day-old wild-type (A, B), morpholino-injected (C, D) and steif mutant embryo (E, F). Embryos (A, C, E) were illuminated at 72 hpf with polarized light to visualize the birefringence in the somitic muscle (for color representation see Fig. 3). Knockdown of Steif/Unc-45b protein (C) by injection of antisense morpholino reduces the birefringence similarly to mutations in steif (E). (B, D, F) wild-type (B), morpholino injected (D) and steif mutant embryos (F) stained immunohistochemically at 48 hpf with the antibody F59 directed against slow muscle myosin. Knockdown embryos (D) and steif (F) mutants have similarly disorganized slow muscle myofibrils. Scale bar: 50 μm (A, C, E); 15 μm (B, D, F).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	unc45b^sb60/sb60 WT + MO3-unc45b
Knockdown Reagent:	MO3-unc45b
Observed In:	eye heart contraction larval locomotory behavior slow muscle cell myosin filament trunk musculature
Stage Range:	Prim-5 to Protruding-mouth

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 308(1), Etard, C., Behra, M., Fischer, N., Hutcheson, D., Geisler, R., and Strähle, U., The UCS factor Steif/Unc-45b interacts with the heat shock protein Hsp90a during myofibrillogenesis, 133-143, Copyright (2007) with permission from Elsevier. Full text @ Dev. Biol.