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ZFIN ID: ZDB-FIG-070314-27
Ma et al., 2007 - Jagged2a-notch signaling mediates cell fate choice in the zebrafish pronephric duct. PLoS Genetics   3(1):e18 Full text @ PLoS Genet.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 14-19 somites to Prim-5

Fig. 5 her9 is a Downstream Target Gene of Jagged2a-Notch1a/Notch3 Signaling(A and B) Compared to (A) wt embryos, her9 expression in the pronephric duct region at 18 ss is severely down-regulated in (B) jagged2a-sp morphants. (C–F) Compared to (C) wt embryos, her9 expression in the pronephric duct region at 17 ss is mildly down-regulated in (D) notch1a (desth35b) mutants and (E) notch3-sp morphants, and is severely down-regulated in (F) notch3-sp MO-injected notch1a (desth35b) mutants. (G and H) Compared to (G) wt embryos, her9 expression in the pronephric duct region at 18 ss is severely down-regulated in (H) mibta52b mutants. (I and J) Coinjection of GFP mRNA (50 pg) and notch1aicd mRNA (100 pg) into one blastomere at the two-cell stage leads to (I) somite boundary disruption in the right half of the embryo, while somites on the left side are segmented properly. (J) GFP expression demonstrates that mRNA is localized to the right half of the embryo. (K) Compared to the left side of the embryo, her9 expression in the duct (arrows) and glomerulus (arrowheads) is increased in the right side at 18 ss. (L and M) Compared to (L) wt embryos, the multi-cilia cell number is increased in (M) her9-utr morphants as shown by rfx2 expression at 24 hpf. All embryos, anterior to the left. (A–K) are dorsal views; (L and M) are lateral views. Bar scale: 100 μm (A–J), 130 μm (K), and 50 μm (L and M).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
her9 WT standard conditions 14-19 somites pronephric duct ISH
14-19 somites pronephric glomerulus ISH
WT + MO6-notch3 standard conditions 14-19 somites pronephric duct ISH
WT + MO7-jag2b standard conditions 14-19 somites pronephric duct ISH
mib1ta52b/ta52b standard conditions 14-19 somites pronephric duct ISH
notch1ath35b/th35b standard conditions 14-19 somites pronephric duct ISH
notch1ath35b/th35b + MO6-notch3 standard conditions 14-19 somites pronephric duct ISH
rfx2 WT standard conditions Prim-5 pronephric duct ISH
WT + MO3-her9 standard conditions Prim-5 pronephric duct ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal PLoS Genetics for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ PLoS Genet.