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ZFIN ID: ZDB-FIG-070307-56
Stoick-Cooper et al., 2007 - Distinct Wnt signaling pathways have opposing roles in appendage regeneration. Development (Cambridge, England)   134(3):479-489 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Condition:
Anatomical Terms:
Stage: Adult
PHENOTYPE:
Fish:
Condition:
Observed In:
Stage: Adult

Fig. 3 Wnt/β-catenin signaling regulates specification and proliferation of the regeneration blastema. (A) Expression of lef1, a marker for the basal epidermal layer of the regeneration epithelium, msxb, marking the mesenchymal progenitor cells of the blastema, and shh, expressed in basal epidermal cells (shown in thick sections), is strongly reduced in Dkk1-overexpressing fins. lef1 is shown at 24 hpa (n=4), msxb (n=4) and shh (n=4) at 72 hpa. Fish were heat shocked twice daily starting shortly before amputation. (B) Hematoxylin-stained sections of tail fin regenerates at 48 hpa. Dkk1-overexpressing fins (right panel; n=6) display reduced numbers of deep mesenchymal cells of the blastema. Fish were heat shocked twice daily starting shortly before amputation. Arrowheads indicate the plane of amputation. (C) 72 hpa regenerates stained for BrdU (red), phosphorylated histone H3 (PH3, green) and DAPI (blue). Cell proliferation in both the mesenchyme and epithelium is decreased in Dkk1-overexpressing fins. Fish were heat shocked once at 66 hpa and fixed at 72 hpa. (D) Quantification of the cell proliferation defects in Dkk1-overexpressing regenerating fins. The fraction of BrdU-positive (left) and PH3-positive (right) cells relative to the total number of cells (DAPI-positive) is shown in percent (n=11). Error bars represent the s.e.m; *P=0.0495; **P=0.0025; ***P=7.076x10-6 (two-tailed).

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
lef1 WT physical alteration: anatomical structure, heat shock Adult regenerating fin ISH
Adult regeneration epithelium ISH
w32Tg/+ physical alteration: anatomical structure, heat shock Adult regenerating fin ISH
Adult regeneration epithelium ISH
msx1b WT physical alteration: anatomical structure, heat shock Adult blastema ISH
Adult regenerating fin ISH
w32Tg/+ physical alteration: anatomical structure, heat shock Adult blastema ISH
Adult regenerating fin ISH
shha WT physical alteration: anatomical structure, heat shock Adult regenerating fin ISH
w32Tg/+ physical alteration: anatomical structure, heat shock Adult regenerating fin ISH
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
w32Tg/+ physical alteration: anatomical structure, heat shock Adult blastema cellular quality, abnormal
Adult blastema hypoplastic, abnormal
Adult blastemal cell decreased amount, abnormal
Adult cell population proliferation decreased rate, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development