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ZFIN ID: ZDB-FIG-070219-33
Nair et al., 2007 - Requirements for Endothelin type-A receptors and Endothelin-1 signaling in the facial ectoderm for the patterning of skeletogenic neural crest cells in zebrafish. Development (Cambridge, England)   134(2):335-345 Full text @ Development
Anatomical Terms:
Stage: Prim-15

Fig. 7 Facial ectoderm is a crucial functional source of Edn1 in the arches. Whole-mount RNA in situ hybridizations at 30 hpf (A,B,D,E), with immunohistochemistry for biotin-dextran (brown cells in C,F-I). Dorsal views (A-C), dorsolateral views (D,E,G-I), 96 hpf flat-mounted Alcian-Blue-stained cartilages (J,K) combined with immunohistochemistry for biotin-dextran (L). (A,D) hand2 expression in ventral cranial NC cells in wild type (arrowheads), which is lost in suc;edn1-/- (B,E, arrows), except in few cells at arch borders. (C) Rescue of hand2 in suc;edn1-/- by unilateral grafting of ectoderm on the left side (arrow). (F) Transverse cryosection through the arch shows unilateral rescue of hand2 (arrowhead) in a suc;edn1-/- embryo adjacent to grafted ectoderm (brown cells, arrow). The control side did not receive any donor ectoderm and shows no rescue of hand2 (gray arrow). (G) Another example of wild-type ectoderm (brown cells, arrow) rescuing hand2 (arrowheads). (H) Control side of embryo in G. (I) Wild-type endoderm (brown cells, arrow) did not rescue hand2. (J) Wild-type cartilages include the mandibular, hyoid and branchial elements including Meckel's cartilage (arrowhead). (K) Meckel's cartilage is reduced in suc;edn1-/- (arrow). (L) suc;edn1-/- mutant that received wild-type ectoderm shows unilateral rescue of the ventral hyosymplectic cartilage (arrowhead) adjacent to the grafted ectoderm (arrow, brown cells). h, heart; nt, neural tube.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
hand2 WT standard conditions Prim-15 heart tube ISH
Prim-15 pharyngeal arch ISH
edn1tf216b/tf216b standard conditions Prim-15 heart tube ISH
Prim-15 Not Detected pharyngeal arch ISH
Antibody Labeling Details No data available
Phenotype Details No data available
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development