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ZIRC
ZFIN ID: ZDB-FIG-070219-29
Nair et al., 2007 - Requirements for Endothelin type-A receptors and Endothelin-1 signaling in the facial ectoderm for the patterning of skeletogenic neural crest cells in zebrafish. Development (Cambridge, England)   134(2):335-345 Full text @ Development
ADDITIONAL FIGURES
PHENOTYPE:
Fish:
Knockdown Reagents:
Observed In:
Stage: Day 4

Fig. 3 Ednra1 is required for jaw joints and patterns ventral cartilage redundantly with Ednra2. Lateral views of 96 hpf larvae (A,D,G,J,M); flat mounts of stained cartilage (B,E,H,K,N); schematics of cartilages (C,F,I,L,O). (A) The jaw protrudes beneath the eye in wild type (arrowhead). (D,G) In Ednra1 or Ednra2 morphants, the jaw is still prominent (arrowhead). (J) In Ednra1;Ednra2 double morphants, the jaw is absent (arrow), reminiscent of suc;edn1-/- (M, arrow). (B,C) Four distinct jaw elements are visible in the anterior two arches: Meckel's (red, V1), palatoquadrate (blue, D1), ceratohyal (red, V2) and hyosymplectic (blue, D2). (E,F) In Ednra1 morphants, all four develop but fuse. (H,I) Ednra2 morphants are indistinguishable from wild-type siblings, whereas in Ednra1;Ednra2 double morphants (K,L), and in suc;edn1-/- (N,O), ventral elements V1 and V2 are lost.

Gene Expression DetailsNo data available
Antibody Labeling Details No data available
Phenotype Details
Fish Conditions Stage Phenotype
WT + MO1-ednrab standard conditions Day 4 hyosymplectic cartilage dorsal region fused with ceratohyal cartilage ventral region, abnormal
Day 4 interhyal cartilage aplastic, abnormal
Day 4 Meckel's cartilage fused with ceratohyal cartilage, abnormal
Day 4 Meckel's cartilage ventral region fused with palatoquadrate cartilage dorsal region, abnormal
Day 4 ventral mandibular arch decreased length, abnormal
WT + MO1-ednrab + MO2-ednraa standard conditions Day 4 ceratohyal cartilage aplastic, abnormal
Day 4 Meckel's cartilage decreased size, abnormal
Day 4 ventral mandibular arch aplastic, abnormal
edn1tf216b/tf216b standard conditions Day 4 ceratohyal cartilage aplastic, abnormal
Day 4 Meckel's cartilage decreased size, abnormal
Day 4 ventral mandibular arch aplastic, abnormal
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development