ZFIN ID: ZDB-FIG-070219-13
Hernandez et al., 2007 - Cyp26 enzymes generate the retinoic acid response pattern necessary for hindbrain development. Development (Cambridge, England)   134(1):177-187 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
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Anatomical Terms:
Stage: 14-19 somites

Fig. 4 cyp26a1 protects the hindbrain from exogenous RA. Wild-type (left column) and cyp26a1-/- (right column) embryos treated with DMSO (A,B), 10 μM DEAB (C,D) or 10 μM DEAB + 5 nM RA (E-L). RNA in situ hybridizations use the markers described in Fig. 2, except for I,J, which is a mix of en3 (bracket), krox20 (r3, r5), dlx2 [cranial neural crest (cnc) and forebrain (fb)] and myoD (somites; s). Large bracket in A indicates the r7-r8 region, which is elongated in cyp26a1 mutants (B). (C) In DEAB-treated embryos, posterior rhombomeres (r5-r8) are absent (arrow indicates the absence of high hoxd4 expression characteristic of r7-r8). (D) This phenotype is partially rescued in cyp26a1 mutants, as seen by rescue of r5 but not of r7-r8. (E-L) The DEAB phenotype is fully rescued in wild-type embryos by treatment with 5 nM RA (E,G,I,K) whereas, in cyp26a1 mutants, this low dose of RA causes strong posteriorization of the brain (F,H,J,L). This phenotype resembles that of wild-type embryos treated with 200 nM RA (inset in J). Scale bar: 100 μm. os: optic stalk; e: eye; p: pronephros.

Gene Expression Details
Gene Antibody Fish Conditions Stage Qualifier Anatomy Assay
dlx2a cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: all-trans-retinoic acid 14-19 somites Not Detected brain ISH
egr2b cyp26a1rw716/rw716 control 14-19 somites rhombomere 3 ISH
14-19 somites rhombomere 5 ISH
cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde 14-19 somites rhombomere 3 ISH
14-19 somites rhombomere 5 ISH
cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: all-trans-retinoic acid 14-19 somites Not Detected brain ISH
en2b cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: all-trans-retinoic acid 14-19 somites Not Detected brain ISH
hoxb1a cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: all-trans-retinoic acid 14-19 somites Not Detected brain ISH
hoxd4a cyp26a1rw716/rw716 control 14-19 somites rhombomere 7 ISH
14-19 somites rhombomere 8 ISH
cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde 14-19 somites Not Detected rhombomere ISH
cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: all-trans-retinoic acid 14-19 somites hindbrain ISH
myod1 cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: all-trans-retinoic acid 14-19 somites somite ISH
otx2b cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: all-trans-retinoic acid 14-19 somites Not Detected brain ISH
pax2a cyp26a1rw716/rw716 control 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites optic stalk ISH
14-19 somites otic vesicle ISH
14-19 somites rhombic lip ISH
cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde 14-19 somites midbrain ISH
14-19 somites midbrain hindbrain boundary ISH
14-19 somites optic stalk ISH
14-19 somites otic vesicle ISH
14-19 somites rhombic lip ISH
cyp26a1rw716/rw716 chemical treatment: 4-(diethylamino)benzaldehyde, chemical treatment: all-trans-retinoic acid 14-19 somites Not Detected brain ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development