Fig. S1

Spatiotemporal expression of myf5, myod, myogenin, α-actin and RFP during cranial muscle development. Embryos derived from the wild-type (A–T) and from the transgenic line Tg(α-actin:RFP) (U–Y) were observed by lateral view except that E, J, O, T, and Y were observed by dorsal view. Comparison the expression patterns of myf5 (A–E), myod (F–J), myogenin (K–O) and α-actin (P–T), we found that the myf5 transcripts in the superior oblique (so) and inferior oblique (io) were gradually decreased during 36 to 48 hpf. The myf5 transcripts were not detected in the head after 58 hpf (A–E). myod was expressed earlier than myogenin and α-actin in the cranial primordial muscle cells (F vs. K and P). The myod transcripts were present in most cranial muscles except io and so at 36 hpf; thereafter, myod was expressed all cranial muscles at 482Fi> in the cranial primordial muscle cells (F vs. K and P). The myod transcripts were present in most cranial muscles except  hpf (G). myogenein was slightly expressed in the intermandibularis, masticatory plate, and io at 36 hpf (K), and were detected in all the cranial muscles similar to myod staining at 48 hpf (G vs. L). Whereas α-actin transcripts did not detectable until 58 hpf (V–W). The RFP expression in the Tg(α-actin:RFP) embryos was detectable at 55 hpf, and fluorescent signal became very prominent at 58 hpf