Fig. 5

Fgfrl1a is essential for gcm2 expression within the pharyngeal epithelium. (A, C and E) Neural crest expression of dlx2 in 23 somite (dorsal view), 26 hpf (dorsolateral view) and 48 hpf (lateral view) wildtype embryos, respectively. (B, D and F) dlx2 expression in 23 somite, 26 hpf and 48 hpf Fgfrl1a morphant embryos, respectively. Neural crest streams are numbered. (G/I and H/J) Lateral views of erm expression within pharyngeal domain of 48 hpf wildtype and Fgfrl1a morphant embryos, respectively. (G/H and I/J) Lateral views and equivalent sagittal sections, respectively. Arrows mark pharyngeal pouches within branchial domain. (K/M and L/N) Lateral views of pea3 expression within pharyngeal domain of 48 hpf wildtype and Fgfrl1a morphant embryos, respectively. (K/L and M/N) Lateral views and equivalent sagittal sections, respectively. Arrows mark pharyngeal pouches within branchial domain. (O and P) Lateral views of gcm2 expression within pharyngeal domain of 48 hpf wildtype and Fgfrl1a morphant embryos, respectively. Inset represents enlarged views of boxed regions. Asterisks mark branchial arches. (Q/S and R/T) sox9b expression within pharyngeal domain of 72 hpf wildtype and Fgfrl1a morphant larvae, respectively. (Q/R and S/T) Lateral views and equivalent frontal sections, respectively. (U and V) Lateral views of Zn5-stained pharyngeal pouch endoderm within 36 hpf wildtype and Fgfrl1a morphant embryos, respectively. (W and X) Lateral views of fgf3 expression within pharyngeal domain of 48 hpf wildtype and Fgfrl1a morphant embryos, respectively. Inset represents enlarged views of boxed regions. Asterisks mark pharyngeal pouches. Abbreviations: B, branchial arches; Ec, pharyngeal ectoderm; En, pharyngeal endoderm; H, hyoid arch; M, mandibular arch; OV, otic vesicle; p1–5, pharyngeal pouches 1–5. Scale bars: 100 μm in A–X.