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ZFIN ID: ZDB-FIG-060929-10
Liu et al., 2006 - Endothelium is required for the promotion of interrenal morphogenetic movement during early zebrafish development. Developmental Biology   297(1):44-58 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagent:
Anatomical Terms:
Stage Range: Long-pec to Protruding-mouth

Fig. 6 Effects of vegf-A antisense morpholino injection on the interrenal tissue. (A, B) Dorsal views of uninjected control (A) and vegf-A morphant (B) embryos after double ISH for wt1 (dark brown) and ff1b (red) at 51 hpf, oriented with anterior to the left. (C–N) Single confocal sections showing the dorsal views of interrenal tissues as detected by 3β-Hsd activity staining (C, F, I, L), and the neighboring ECs as labeled by green fluorescence (D, G, J, M), of 54 hpf (C–H) or 80 hpf (I–N) Tg(fli1:EGFP)Y1 embryos injected with STD-MO (C–E, I–K) and vegfMO (F–H, L–N), respectively and oriented with anterior to the top. The merged images of 3β-Hsd activity and GFP are shown in panels E, H, K and N. The convergence of pronephric but not interrenal tissues is perturbed in vegf-A morphants. PCV, posterior cardinal vein. Red arrows indicate interrenal tissues, while yellow arrowheads indicate glomeruli. Scale bar, 50 μM.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
hsd3b y1Tg control Long-pec interrenal primordium OTHR
Protruding-mouth interrenal gland OTHR
y1Tg + MO1-vegfaa standard conditions Long-pec interrenal primordium OTHR
Protruding-mouth interrenal gland OTHR
nr5a1a WT standard conditions Long-pec interrenal primordium ISH
WT + MO1-vegfaa standard conditions Long-pec interrenal primordium ISH
wt1a WT standard conditions Long-pec pronephros ISH
WT + MO1-vegfaa standard conditions Long-pec pronephros ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 297(1), Liu, Y.W., and Guo, L., Endothelium is required for the promotion of interrenal morphogenetic movement during early zebrafish development, 44-58, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.