FIGURE

Fig. 5

ID
ZDB-FIG-060609-3
Publication
Connors et al., 2006 - Temporal and spatial action of Tolloid (Mini fin) and Chordin to pattern tail tissues
Other Figures
All Figure Page
Back to All Figure Page
Fig. 5

Chordin activity is required during gastrulation and segmentation stages. (A) Non-transgenic mfn and (B) Tg(hsp70:tld); mfn embryo heat-shocked twice for 1 h, at high stage (∼3.5 hpf) and then early gastrula stage (∼6.5 hpf) (2× early). (C) Representative Tg(hsp70:tld); mfn embryo heat-shocked for 6 rounds from pregastrulation to the 18-somite stage (6× early, N = 10). Note the stronger ventralization in the embryo lacking Chd activity during both gastrulation and somitogenesis (C). Non-transgenic (D) and Tg(hsp70:tld) (E–F) mfn embryos heat-shocked 4 times between the bud and 18-somite stages. A weak ventralized phenotype is observed as excess blood (arrow), blistering of the ventral tail fin (arrowhead in panel E), and small duplications of the ventral tail fin (arrowhead in panel F). krox20 expression in non-transgenic (G) and transgenic (H) mfn embryos heat-shocked 3 times prior to and during gastrulation (N = 22). krox20 expression in non-transgenic (I) and transgenic (J) mfn embryos (N = 20) at the 9-somite stage that were heat-shocked twice after gastrulation (2× late). A 2× rather than 4× heat-shock was performed so that krox20 expression could be examined at the 9-somite stage. Note a reduction in krox20 expression only when Chd is inhibited during gastrulation.

Expression Data
Gene:
Fish:
Condition:
Anatomical Terms:
Stage: 5-9 somites

Expression Detail
Antibody Labeling
Phenotype Data

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Biology, 293(1), Connors, S.A., Tucker, J.A., and Mullins, M.C., Temporal and spatial action of Tolloid (Mini fin) and Chordin to pattern tail tissues, 191-202, Copyright (2006) with permission from Elsevier. Full text @ Dev. Biol.