ZFIN ID: ZDB-FIG-050608-3
Oates et al., 2005 - Cooperative function of deltaC and her7 in anterior segment formation. Developmental Biology   280(1):133-149 Full text @ Dev. Biol.
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Gene:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 1-4 somites to 10-13 somites

Fig. 4 Transcriptional activity of the deltaC gene in wild type and segmentation defective mutant backgrounds. Distribution of total dlc mRNA, detected with a cDNA-derived riboprobe, is shown in panels (C–F) and (I). Distribution of unspliced dlc pre-mRNA, detected with a riboprobe to the fourth intron, is shown in panels (A), (B), (G), (H), and (J–M). Dorsal view in flat mounted embryos in wild type (A–E), morpholino injected (F–I.M), and mutant (J, aei/dld; K des/n1a,; L, bea) backgrounds. Embryos are shown at bud (E, F), 3 (A, G), 7 (D), and 10 somite (B, C, H, I, J–M) stages of development. Panels (F–I) show the effect of injection of 1 ng/nL dlcMO3 and 2 ng/nL dlcMO4, and panel (M) shows the effect of 2 ng/nL her7MO1 and her7MO2. The total distribution of dlc mRNA in a representative embryo, as detected with the exonic riboprobe by confocal LSM, is shown in panel D, with (a) anterior, (b) mid-, and (c) posterior wavelike expression domains, (b) and is magnified in right panel showing puncta (arrows) on the rostral side, and diffuse staining (asterisks) in the caudal part of the expression domain.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
dlc WT standard conditions Bud presumptive segmental plate ISH
1-4 somites segmental plate ISH
Bud tail bud ISH
1-4 somites tail bud ISH
5-9 somites segmental plate ISH
5-9 somites tail bud ISH
10-13 somites segmental plate ISH
10-13 somites tail bud ISH
WT + MO3-dlc + MO4-dlc standard conditions Bud presumptive segmental plate ISH
1-4 somites segmental plate ISH
1-4 somites tail bud ISH
Bud tail bud ISH
10-13 somites segmental plate ISH
10-13 somites tail bud ISH
dlctm98/tm98 standard conditions 10-13 somites notochord ISH
10-13 somites segmental plate ISH
10-13 somites tail bud ISH
dldtr233/tr233 standard conditions 10-13 somites notochord ISH
10-13 somites segmental plate ISH
10-13 somites tail bud ISH
notch1atp37/tp37 standard conditions 10-13 somites segmental plate ISH
10-13 somites tail bud ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Developmental Biology for permission to reproduce figures from this article. Please note that this material may be protected by copyright.

Reprinted from Developmental Biology, 280(1), Oates, A.C., Mueller, C., and Ho, R.K., Cooperative function of deltaC and her7 in anterior segment formation, 133-149, Copyright (2005) with permission from Elsevier. Full text @ Dev. Biol.