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ZFIN ID: ZDB-FIG-050505-9
Bae et al., 2005 - Patterning of proneuronal and inter-proneuronal domains by hairy- and enhancer of split-related genes in zebrafish neuroectoderm. Development (Cambridge, England)   132(6):1375-1385 Full text @ Development
ADDITIONAL FIGURES
EXPRESSION / LABELING:
Genes:
Fish:
Knockdown Reagents:
Anatomical Terms:
Stage Range: 1-4 somites to Prim-15

Fig. 5 Her3 and Her9 repress the expression of proneural genes. (A-H) Expression of neurod4 in the wild-type control embryos (A,E), embryos that received an injection of 2 ng her9ATG-MO (H9MO; B,F), mib mutant embryos (C,G) and mib mutant embryos that received an injection of her9ATG-MO (D,H) at the one-somite stage. Dorsal views (A-D) and dorsolateral views (E-H). neurod4 was expressed in the inter-proneuronal domain between the RB neurons and primary interneurons in the her9ATG-MO-injected wild-type and mib mutant embryos, where neurod4 was not expressed in the control embryos. (I-L) The her9-MO-injected embryos had a reduced and narrow fourth ventricle at 30 hours post fertilization (hpf) (J,L), compared with the control embryos (I,K). atoh1 marks neuronal precursor cells located in the subventricular zone of the fourth ventricle. Lateral views (I,J) and dorsal views of the hindbrain region (K,L). (M-N) Expression of neurog1 in 1 ng of control MO (M) and her3-MO1-injected embryos (H3MO; N) at the one-somite stage. Dorsal views for the anterior hindbrain. Ectopic expression of neurog1 was detected only in rhombomeres 2 and 4 of the her3 morphant embryos. (O-R) Her9 functions as a transcriptional repressor. RNAs for Myc-tagged Her9 (Her9MT, 5 pg; O,P), Her9-VP16 fusion protein (Her9VP16, 5 pg; Q) or Myc-tagged Her3 (Her3MT, 20 pg; R) together with 50 pg ß-galactosidase RNA, were injected into one blastomere of two- to four-cell stage embryos. The embryos were fixed at the one-cell stage, and stained with X-gal and the neurod4 probe. In the majority of the her9MT RNA-injected embryos, cells that received her9MT RNA were distributed to only the inter-proneuronal domains (O). However, when the cells were located in the proneuronal domains, the neurod4 expression was reduced or abrogated (P). Expression of Her9VP16 led to an ectopic but spotty expression of neurod4 in the neural plate (Q). Expression of Her3MT led reduction of the neurod4 expression in the proneuronal domains (R). Quantification of the results is shown in Table 1.

Gene Expression Details
Gene Antibody Fish Conditions Stage Anatomy Assay
atoh1a WT standard conditions Prim-15 fourth ventricle ISH
Prim-15 ventricular zone ISH
WT + MO1-her9 standard conditions Prim-15 fourth ventricle ISH
Prim-15 ventricular zone ISH
neurod4 WT + MO1-her9 standard conditions 1-4 somites neural plate ISH
1-4 somites primary neuron ISH
mib1m178/m178 + MO1-her9(AB) standard conditions 1-4 somites neural plate ISH
1-4 somites primary neuron ISH
neurog1 WT standard conditions 1-4 somites hindbrain neural plate ISH
WT + MO3-her3 standard conditions 1-4 somites hindbrain neural plate ISH
Antibody Labeling Details No data available
Phenotype Details No data available
Acknowledgments:
ZFIN wishes to thank the journal Development (Cambridge, England) for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Full text @ Development