EST

cb511

ID
ZDB-EST-021126-22
Name
cb511
Symbol
cb511
Previous Names
None
Type
EST
Location
Unmapped
Genome Resources
None
Species
Danio rerio
Library
oligo-dT primed segmentation stage
Digest
NotI
Insert Size
1200
Cloning Site
EcoRI-SalI Digest
Vector Type
Plasmid
Vector
pSPORT1
Polymerase
T3 RNA polymerase
PCR Amplification
(replaces the SP6 promoter with a T3 promoter) T3p : 5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3' T7p : 5' TAA TAC GAC TCA CTA TAG GG 3'
Suppliers
Quality
( Nice strong expression pattern )
Protocol
Thisse in situ hybridization protocol
Note
The probe available from ZIRC is a 1200 nt subclone of AF371368 in the pSport vector and should be amplified using PCR.
Gene Expression
Directly Submitted Expression Data
7 figures (49 images) from Thisse et al., 2001 [cb511]
Marker Relationships
Sequences
Citations