EST

cb314

ID
ZDB-EST-020816-18
Name
cb314
Symbol
cb314
Previous Names
None
Type
EST
Location
Unmapped
Genome Resources
None
Species
Danio rerio
Library
oligo-dT primed 26-somite stage
Digest
PCR amplification
Insert Size
1000
Cloning Site
NotI-SalI
Vector Type
Plasmid
Vector
pSPORT1
Polymerase
T3 RNA polymerase
PCR Amplification
5' GGA TCC ATT AAC CCT CAC TAA AGG GAA GAG CTA TGA CGT CGC AT 3'
5' TAA TAC GAC TCA CTA TAG GG 3'
Suppliers
Quality
( Simple to use, intense expression pattern restricted to a few structures )
Protocol
Thisse in situ hybridization protocol
Note
Due to the poor incorporation of DIG-NTP by the SP6 RNA polymerase, antisense RNA probes are synthesized from a PCR amplified fragment in which a T3 RNA polymerase replace the SP6 RNA polymerase promoter.
Gene Expression
Directly Submitted Expression Data
6 figures (14 images) from Thisse et al., 2001 [cb314]
Marker Relationships
Sequences
Citations