CRISPR

CRISPR2-foxc1b

ID

ZDB-CRISPR-190103-11

Name

CRISPR2-foxc1b

Previous Names

None

Target

foxc1b (1)

Sequence

5' - GGCGTTGTGCCTTATATCCC - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

None

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
el620	foxc1b
ua1018	foxc1b

Expression

Gene expression in Wild Types + CRISPR2-foxc1b

No data available

Phenotype

Phenotype resulting from CRISPR2-foxc1b

No data available

Phenotype of all Fish created by or utilizing CRISPR2-foxc1b

Phenotype	Fish	Conditions	Figures
determination of heart left/right asymmetry disrupted, abnormal	foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 3 from Chrystal et al., 2021
heart myl7 expression spatial pattern, abnormal	foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 3 from Chrystal et al., 2021
whole organism col11a2 expression increased amount, abnormal	foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 7 from Hawkey-Noble et al., 2022
neural crest pdgfra expression decreased amount, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. S2 from Umali et al., 2019
solid lens vesicle pdgfra expression decreased amount, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. S2 from Umali et al., 2019
retinal ganglion cell layer pou4f2 expression decreased amount, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. 4 from Umali et al., 2019
retina atoh7 expression absent, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. 4 from Umali et al., 2019
cranial nerve II decreased diameter, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. 3 from Umali et al., 2019
retina atoh7 expression decreased amount, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. 4 from Umali et al., 2019
pharyngeal arch pdgfra expression decreased amount, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. S2 from Umali et al., 2019
retinal ganglion cell neuron differentiation decreased occurrence, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. 4 from Umali et al., 2019
retinal ganglion cell layer has fewer parts of type retinal ganglion cell, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. 2 from Umali et al., 2019
head fgf19 expression decreased amount, abnormal	foxc1b^{ua1018/ua1018} + MO2-foxc1a (AB)	standard conditions	Fig. 5 from Umali et al., 2019
symplectic decreased size, abnormal	foxc1a^el543/+; foxc1b^el620/el620	standard conditions	Fig. 2 from Xu et al., 2018
palatoquadrate cartilage decreased size, abnormal	foxc1a^el543/el543; foxc1b^el620/+	standard conditions	Fig. 2 from Xu et al., 2018
symplectic absent, abnormal	foxc1a^el543/el543; foxc1b^el620/+	standard conditions	Fig. 2 from Xu et al., 2018
palatoquadrate cartilage matn1 expression decreased amount, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
palatoquadrate cartilage matn1 expression decreased distribution, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
pharyngeal arch 1 cartilage development decreased occurrence, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 2 , Fig. 6 from Xu et al., 2018
hyosymplectic cartilage matn1 expression decreased distribution, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
hyosymplectic cartilage col2a1a expression decreased distribution, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
hyosymplectic cartilage matn1 expression decreased amount, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
pharyngeal arch cartilage morphology, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 6 from Xu et al., 2018
head decreased size, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 2 from Xu et al., 2018
symplectic absent, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 2 from Xu et al., 2018
hyomandibula decreased size, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 2 from Xu et al., 2018
pharyngeal arch cartilage decreased size, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 6 from Xu et al., 2018
hyosymplectic cartilage acana expression decreased distribution, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
palatoquadrate cartilage matn4 expression decreased distribution, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
neurocranium cartilage development decreased occurrence, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 2 from Xu et al., 2018
palatoquadrate cartilage acana expression decreased distribution, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
pharyngeal arch 2 cartilage development decreased occurrence, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 2 , Fig. 6 from Xu et al., 2018
neurocranial trabecula decreased size, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 2 from Xu et al., 2018
palatoquadrate cartilage decreased size, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 2 from Xu et al., 2018
hyosymplectic cartilage matn4 expression decreased distribution, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
palatoquadrate cartilage col2a1a expression decreased distribution, abnormal	foxc1a^el543/el543; foxc1b^el620/el620	standard conditions	Fig. 5 from Xu et al., 2018
heart edematous, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^ua1018/+	standard conditions	Fig. S11 from Whitesell et al., 2019
ventral aorta vascular associated smooth muscle cell decreased amount, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^ua1018/+; ca7Tg/+; ci5Tg/+	standard conditions	Fig. 8 from Whitesell et al., 2019
aortic arch myl9a expression decreased amount, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018}	standard conditions	Fig. 8 from Whitesell et al., 2019
heart edematous, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018}	standard conditions	Fig. S11 from Whitesell et al., 2019
vascular associated smooth muscle cell foxc1b expression decreased amount, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018}	standard conditions	Fig. 8 from Whitesell et al., 2019
pericyte pdgfrb expression decreased amount, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018}	standard conditions	Fig. 8 from Whitesell et al., 2019
vascular associated smooth muscle cell foxc1a expression decreased amount, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018}	standard conditions	Fig. 8 from Whitesell et al., 2019
vascular associated smooth muscle cell acta2 expression decreased amount, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018}	standard conditions	Fig. 8 from Whitesell et al., 2019
heart myl7 expression spatial pattern, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 3 from Chrystal et al., 2021
brain ventricular system swollen, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 2 from Chrystal et al., 2021
head deformed, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 2 from Chrystal et al., 2021
determination of pancreatic left/right asymmetry disrupted, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 3 from Chrystal et al., 2021
determination of heart left/right asymmetry disrupted, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 3 from Chrystal et al., 2021
brain hemorrhagic, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 2 from Chrystal et al., 2021
pericardium edematous, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 4 from Hawkey-Noble et al., 2022 Figure 2 from Chrystal et al., 2021
opercular flap ossification decreased process quality, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 4 from Hawkey-Noble et al., 2022
determination of liver left/right asymmetry disrupted, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 3 from Chrystal et al., 2021
lateral plate mesoderm lft2 expression spatial pattern, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 6 from Chrystal et al., 2021
pancreas foxa3 expression spatial pattern, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 3 from Chrystal et al., 2021
pharyngeal arch 1 ossification decreased process quality, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 4 from Hawkey-Noble et al., 2022
eye decreased size, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 2 from Chrystal et al., 2021
pharyngeal arch 2 ossification decreased process quality, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 4 from Hawkey-Noble et al., 2022
liver foxa3 expression spatial pattern, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018} (AB)	standard conditions	Figure 3 from Chrystal et al., 2021
ventral aorta vascular associated smooth muscle cell decreased amount, abnormal	foxc1a^{ua1017/ua1017}; foxc1b^{ua1018/ua1018}; ca7Tg/+; ci5Tg/+	standard conditions	Fig. 8 from Whitesell et al., 2019
cranial cartilage ossification absent process, abnormal	foxc1b^{ua1018/ua1018}; foxl1^nfl1/nfl1 (AB)	standard conditions	Figure 6 from Hawkey-Noble et al., 2022
vertebra ossification absent process, abnormal	foxc1b^{ua1018/ua1018}; foxl1^nfl1/nfl1 (AB)	standard conditions	Figure 6 from Hawkey-Noble et al., 2022
coelom edematous, abnormal	foxc1b^{ua1018/ua1018}; foxl1^nfl1/nfl1 (AB)	standard conditions	Figure 6 from Hawkey-Noble et al., 2022
symplectic chondrocyte mCherry expression decreased amount, abnormal	foxc1a^el543/el543; foxc1b^el620/el620; b1212Tg; el559Tg	standard conditions	Fig. S6 from Xu et al., 2018
hyosymplectic cartilage chondrocyte mCherry expression decreased amount, abnormal	foxc1a^el543/el543; foxc1b^el620/el620; b1212Tg; el559Tg	standard conditions	Fig. S6 from Xu et al., 2018
palatoquadrate cartilage chondrocyte mCherry expression decreased amount, abnormal	foxc1a^el543/el543; foxc1b^el620/el620; b1212Tg; el559Tg	standard conditions	Fig. S6 from Xu et al., 2018
pharyngeal arch cartilage morphology, ameliorated	foxc1a^el543/el543; foxc1b^el620/el620; el360Tg; el700Tg	standard conditions	Fig. 6 from Xu et al., 2018
pharyngeal arch cartilage size, ameliorated	foxc1a^el543/el543; foxc1b^el620/el620; el360Tg; el700Tg	standard conditions	Fig. 6 from Xu et al., 2018

Citations