CRISPR

CRISPR2-wwtr1

ID

ZDB-CRISPR-181210-4

Name

CRISPR2-wwtr1

Previous Names

None

Target

wwtr1 (1)

Sequence

5' - GGAGTCTCCCGGGGCT - 3'

Disclaimer

Although ZFIN verifies reagent sequence data, we recommend that you conduct independent sequence analysis before ordering any reagent.

Note

The PAM sequence was "CGG" at the 3' end.

Genome Resources

None

Target Location

Constructs

No data available

Genomic Features

Genomic Feature	Affected Genomic Regions
swu46	wwtr1
swu47	wwtr1

Expression

Gene expression in Wild Types + CRISPR2-wwtr1

No data available

Phenotype

Phenotype resulting from CRISPR2-wwtr1

No data available

Phenotype of all Fish created by or utilizing CRISPR2-wwtr1

Phenotype	Fish	Conditions	Figures
whole organism wwtr1 expression decreased amount, abnormal	wwtr1^swu46/swu46	standard conditions	Fig. 2 from Yi et al., 2018
swim bladder decreased size, abnormal	wwtr1^swu46/swu46 (AB/TU)	standard conditions	Fig. 1 from Yi et al., 2019
chorion micropyle formation decreased occurrence, abnormal	wwtr1^swu46/swu46 (AB/TU)	standard conditions	Fig. 5 from Yi et al., 2019
micropylar cell wwtr1 expression absent, abnormal	wwtr1^swu46/swu46 (AB/TU)	standard conditions	Fig. 5 from Yi et al., 2019
whole organism wwtr1 expression absent, abnormal	wwtr1^swu46/swu46 (AB/TU)	standard conditions	Fig. 1 from Yi et al., 2019
pericardium edematous, abnormal	wwtr1^swu46/swu46 (AB/TU)	standard conditions	Fig. 1 from Yi et al., 2019
fertilized egg chrorion micropyle absent, abnormal	wwtr1^swu46/+ (AB/TU)	standard conditions	Fig. 4 from Yi et al., 2019
embryo development arrested, abnormal	wwtr1^swu46/+ (AB/TU)	standard conditions	Fig. 2 from Yi et al., 2019
fertilization decreased occurrence, abnormal	wwtr1^swu46/+ (AB/TU)	standard conditions	Fig. 4 from Yi et al., 2019
fertilized egg pronucleus decreased amount, abnormal	wwtr1^swu46/+ (AB/TU)	standard conditions	Fig. 4 from Yi et al., 2019
chorion micropyle formation decreased occurrence, abnormal	wwtr1^swu46/+ (AB/TU)	standard conditions	Fig. 4 from Yi et al., 2019
embryo development arrested, abnormal	wwtr1^swu47/+ (AB/TU)	standard conditions	Fig. 2 from Yi et al., 2019
intestine decreased size, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
intestine fabp2 expression decreased distribution, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
liver decreased size, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
intestinal rod endodermal cell distributed, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
liver fabp1a expression decreased amount, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
liver fabp1a expression spatial pattern, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
intestine morphology, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
exocrine pancreas prss1 expression decreased distribution, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
liver mislocalised, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
lateral plate mesoderm mislocalised, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 4 from Yi et al., 2018
liver split bilaterally, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
intestine fabp2 expression decreased amount, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
liver prox1a expression decreased amount, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
exocrine pancreas prss1 expression spatial pattern, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
exocrine pancreas split bilaterally, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
liver prox1a expression increased distribution, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
liver prox1a expression spatial pattern, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
exocrine pancreas prss1 expression decreased amount, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
exocrine pancreas decreased size, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 3 from Yi et al., 2018
liver cell population proliferation decreased occurrence, abnormal	wwtr1^swu46/+; yap1^swu45/swu45	standard conditions	Fig. 4 from Yi et al., 2018
trunk morphology, abnormal	wwtr1^swu46/swu46; yap1^swu45/swu45	standard conditions	Fig. 2 from Yi et al., 2018
caudal fin curved dorsal, abnormal	wwtr1^swu46/swu46; yap1^swu45/swu45	standard conditions	Fig. 2 from Yi et al., 2018
developmental process delayed, abnormal	wwtr1^swu46/swu46; yap1^swu45/swu45	standard conditions	Fig. 2 from Yi et al., 2018
trunk decreased length, abnormal	wwtr1^swu46/swu46; yap1^swu45/swu45	standard conditions	Fig. 2 from Yi et al., 2018
whole organism morphology, abnormal	wwtr1^swu46/swu46; yap1^swu45/swu45	standard conditions	Fig. 2 from Yi et al., 2018
whole organism decreased length, abnormal	wwtr1^swu46/swu46; yap1^swu45/swu45	standard conditions	Fig. 2 from Yi et al., 2018

Citations