cDNA

MGC:73031

ID
ZDB-CDNA-040425-426
Name
MGC:73031
Symbol
MGC:73031
Previous Names
  • FR_5351 (1)
Type
cDNA
Location
Unmapped
Genome Resources
None
Species
Danio rerio
Library
Zebrafish adult retina cDNA
Digest
Insert Size
2143
Cloning Site
EcoRI-SalI
Vector Type
Plasmid
Vector
pBluescript SK-
Polymerase
T7 RNA polymerase
PCR Amplification
Reaction denatured 4 min. followed by PCR cycling 95°C 30s, 55°C 30s, 72°C 3 min. (at least 1 min. per kb) followed by elongation at 72°C 7 min.
5' AAC AGC TAT GAC CAT G 3'
5' GTA AAA CGA CGG CCA GT 3'
Suppliers
None
Quality
( Simple to use, intense expression pattern restricted to a few structures )
Protocol
Thisse in situ hybridization protocol
Note
None
Gene Expression
Directly Submitted Expression Data
6 figures (12 images) from Thisse et al., 2004 [MGC:73031]
Marker Relationships
Sequences
Citations